FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26976
Total Bases4.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2490.9230427046263345No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2440.9045077105575327No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1960.7265717674970344No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1180.43742586002372474No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1160.43001186239620404No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1090.4040628706998814No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA950.35216488730723605No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT940.3484578884934757No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA870.322508896797153No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT800.29655990510083036No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA760.28173190984578883No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC760.28173190984578883No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA750.2780249110320285No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT750.2780249110320285No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT700.25948991696322654No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA680.25207591933570583No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC670.24836892052194542No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG630.2335409252669039No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC610.22612692763938316No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC610.22612692763938316No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG580.21500593119810202No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC550.20388493475682087No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG550.20388493475682087No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC530.19647093712930014No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG530.19647093712930014No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC530.19647093712930014No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG520.19276393831553976No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC500.185349940688019No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT500.185349940688019No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT490.1816429418742586No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT480.1779359430604982No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA470.17422894424673785No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG460.17052194543297747No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT460.17052194543297747No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT450.16681494661921709No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC450.16681494661921709No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA440.1631079478054567No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT430.15940094899169632No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG430.15940094899169632No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG420.15569395017793594No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG420.15569395017793594No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG410.15198695136417556No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG410.15198695136417556No Hit
CTTCTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGC410.15198695136417556No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG400.14827995255041518No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT400.14827995255041518No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA400.14827995255041518No Hit
GCCAAAGCTAAGACCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAA390.1445729537366548No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG390.1445729537366548No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA390.1445729537366548No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC380.14086595492289442No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG380.14086595492289442No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT370.13715895610913403No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT370.13715895610913403No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC370.13715895610913403No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG360.13345195729537368No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT360.13345195729537368No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC350.12974495848161327No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA350.12974495848161327No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT340.12603795966785292No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG340.12603795966785292No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC340.12603795966785292No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA340.12603795966785292No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG340.12603795966785292No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA330.12233096085409252No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC330.12233096085409252No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT330.12233096085409252No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC330.12233096085409252No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA320.11862396204033215No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC320.11862396204033215No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC320.11862396204033215No Hit
CATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATA320.11862396204033215No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT310.11491696322657177No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT310.11491696322657177No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT310.11491696322657177No Hit
CCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCA310.11491696322657177No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA310.11491696322657177No Hit
ACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTC300.1112099644128114No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG300.1112099644128114No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA290.10750296559905101No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT290.10750296559905101No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG290.10750296559905101No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA290.10750296559905101No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC290.10750296559905101No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT290.10750296559905101No Hit
GGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGA290.10750296559905101No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA290.10750296559905101No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG290.10750296559905101No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA280.10379596678529061No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG280.10379596678529061No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA280.10379596678529061No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA280.10379596678529061No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC280.10379596678529061No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC280.10379596678529061No Hit
CAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAGCCCT280.10379596678529061No Hit
GTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA280.10379596678529061No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC280.10379596678529061No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT270.10008896797153025No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA270.10008896797153025No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC270.10008896797153025No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT270.10008896797153025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)