FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39460
Total Bases6.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1790.45362392295995946No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1480.37506335529650275No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1440.3649265078560568No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1380.34972123669538774No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1340.3395843892549417No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1030.2610238215914851No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC960.2432843385707045No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA960.2432843385707045No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT940.2382159148504815No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG910.230613279270147No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA900.2280790674100355No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA870.22047643182970095No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC850.21540800810947794No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA770.1951343132285859No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT770.1951343132285859No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA750.1900658895083629No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA750.1900658895083629No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC740.18753167764825138No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA720.1824632539280284No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC700.1773948302078054No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA700.1773948302078054No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA660.16725798276735934No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA650.16472377090724785No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT640.16218955904713633No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG600.15205271160669032No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG590.14951849974657883No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC550.1393816523061328No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT540.1368474404460213No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC530.13431322858590977No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG520.13177901672579828No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA520.13177901672579828No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC510.12924480486568676No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT500.12671059300557527No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT500.12671059300557527No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG490.12417638114546375No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA490.12417638114546375No Hit
CTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTGGAT490.12417638114546375No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC490.12417638114546375No Hit
CCCTAGCATTACTTATATGATATGTCTCCATACCCATTACAATCTCCAGC480.12164216928535225No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG480.12164216928535225No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT470.11910795742524075No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT470.11910795742524075No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA460.11657374556512926No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT450.11403953370501775No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA440.11150532184490625No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA440.11150532184490625No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT430.10897110998479473No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT430.10897110998479473No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC430.10897110998479473No Hit
GTACCTAACAAACCCACAGGTCCTAAACTACCAAACCTGCATTAAAAATT430.10897110998479473No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT420.10643689812468322No Hit
CCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC420.10643689812468322No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC420.10643689812468322No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA420.10643689812468322No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG420.10643689812468322No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC420.10643689812468322No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC410.10390268626457172No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTAC410.10390268626457172No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA410.10390268626457172No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA400.10136847440446022No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCGACCTCGG400.10136847440446022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)