FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008160957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008160957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41963
Total Bases5.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3050.7268307794962229No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2860.6815527965112123No Hit
GGTATCAACGCAGAGTACATGGGGG2810.6696375378309463No Hit
GTATCAACGCAGAGTACATGGGGGG2680.6386578652622549No Hit
GTATCAACGCAGAGTACATGGGGGGG1900.452779829850106No Hit
TATCAACGCAGAGTACATGGGGGGG1620.3860543812406167No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1520.3622238638800848No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1230.2931153635345424No Hit
GTATCAACGCAGAGTACATGGGGAG1080.2573695874937445No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1010.2406882253413722No Hit
GTATCAACGCAGAGTACATGGGGTG960.2287729666611062No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT960.2287729666611062No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT930.22162381145294663No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA890.21209160450873388No Hit
GTATCAACGCAGAGTACATGGGATG880.2097085527726807No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC820.19541024235636156No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG770.1834949836760956No Hit
GGTATCAACGCAGAGTACATGGGGGG750.17872888020398922No Hit
GGTATCAACGCAGAGTACATGGGGTG710.16919667325977647No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC690.1644305697876701No Hit
GTATCAACGCAGAGTACATGGGAGG610.14536615589924456No Hit
GGTATCAACGCAGAGTACATGGGGGGG560.13345089721897863No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT540.12868479374687225No Hit
GTATCAACGCAGAGTACATGGAGAG510.12153563853871269No Hit
GGTATCAACGCAGAGTACATGGGGAG500.11915258680265947No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC500.11915258680265947No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG490.11676953506660628No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC490.11676953506660628No Hit
TATCAACGCAGAGTACATGGGGGGGACCTCC480.1143864833305531No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG480.1143864833305531No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA460.10962037985844672No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA460.10962037985844672No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG450.10723732812239353No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA450.10723732812239353No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC450.10723732812239353No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA450.10723732812239353No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT430.10247122465028716No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT420.10008817291423397No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG420.10008817291423397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)