FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008161048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008161048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47249
Total Bases6.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2530.5354610679591102No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2470.5227623865055345No Hit
GGTATCAACGCAGAGTACATGGGGG1890.40000846578763566No Hit
GTATCAACGCAGAGTACATGGGGGG1420.3005354610679591No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1420.3005354610679591No Hit
GTATCAACGCAGAGTACATGGGGGGG1330.28148743888759553No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1250.2645558636161612No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1210.25609007598044403No Hit
TATCAACGCAGAGTACATGGGGGGG1020.2158775847107875No Hit
GTATCAACGCAGAGTACATGGGGAG1010.21376113780185824No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA930.1968295625304239No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT920.19471311562149463No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA900.19048022180363605No Hit
GTATCAACGCAGAGTACATGGGGTG800.16931575271434315No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA730.15450062435183812No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA720.15238417744290886No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC710.15026773053397954No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC710.15026773053397954No Hit
GTATCAACGCAGAGTACATGGGATG700.14815128362505026No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA700.14815128362505026No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA660.1396854959893331No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT660.1396854959893331No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA650.13756904908040382No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT640.13545260217147453No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG620.13121970835361596No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC590.12487036762682809No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA580.12275392071789878No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT550.11640457999111092No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA550.11640457999111092No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC540.11428813308218162No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG530.11217168617325235No Hit
GGTATCAACGCAGAGTACATGGGGGGG520.11005523926432305No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA520.11005523926432305No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC520.11005523926432305No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG500.10582234544646447No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG500.10582234544646447No Hit
GGTATCAACGCAGAGTACATGGGGGG490.10370589853753519No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT480.10158945162860589No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)