FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008161067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008161067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1061
Total Bases168.7 kbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAAAGCTGGGAACGAGAGGTTAAGCAGCTGCAGGAGGAAACGCCACCT121.1310084825636193No Hit
AGGGAGCTCAGTCTGGTAATGAAAAGAAGAAAATAAAACAAACAACAAAC100.9425070688030159No Hit
CTTTGCTTGTTTGATGAGAGCCCGGTTTGAAGAACATAAGAATGAAAAGG100.9425070688030159No Hit
CTACAGTACAAGCTGCTGGAGCCAGTTCTGCTTCTCGGCAAGGAGCGATT90.8482563619227144No Hit
GGTAGTCGGAGGTTGCAGTGAGCCAATATTGCACCACTGCACTCCAGCCT90.8482563619227144No Hit
TACCAAGAAAGTCGGGATCGTCGGTAAATACGGGACCCGCTATGGGGCCT90.8482563619227144No Hit
CAGCTAGAAATGCTCAAGAACCAGCTGGACCAGATGTATGTCCCTGGGAA90.8482563619227144No Hit
CAATAAGAGGCCGGGTACAGTGGCTCATGCCTGTAATCCCAGCACTTTGG90.8482563619227144No Hit
GAAGGATGTTCCCAGAGGCCCCTGAGGAAGCTCAAAGCCGAACTTGTACT80.7540056550424128No Hit
TTGTATTTCTCTTTGCATTGATGGTTAACATTTTTTTGAGCCAACATTTA80.7540056550424128No Hit
CACACACACAGAAGATTTCAATATAAGAAAACACAGAACTTTGTGAGAGT70.6597549481621112No Hit
GACTAGGACTGCTCCAGAGGCGTGGTCTTAACGTTGTTCTTTTCCCCTGG70.6597549481621112No Hit
AATGAGAAGAGTTAAAAAATAGCATTCTAATCACTAGCTAGGCATAGTTA70.6597549481621112No Hit
GTATGCAACGTTTCATCAAAATTGATGGCAAGGTTCGAGTGGATGTCACA70.6597549481621112No Hit
ACCATATTATGTTACAGATAATACAGGAAAAACGGGGTTACTAGTAGCCG60.5655042412818096No Hit
CAAATAATATTTATTTTTAAACAACGGTTATAGAACTGAAAAGATTTTAA60.5655042412818096No Hit
GAGTCACCATCATGCCCAAAGACATCCAGTTGGCTCGCCGGATACGGGGA60.5655042412818096No Hit
GCATACAGGAAACTGTCCTAAAGGAAAAAGAAAACCACTTTCATTGTTTG60.5655042412818096No Hit
CCTTTAAGTTTCTACAAGAAGGAAAAATCACCAGGATTATTCTACAAGAA60.5655042412818096No Hit
TCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCGCCTTAAGTGAGGAGT60.5655042412818096No Hit
GAGTAAGACATAAAGTTAGTGTCACACCCTGGATTATATTCCACATCTCC60.5655042412818096No Hit
GGGGCTGCAGAACAAATCAAGCACATCCTTGCTAATTTCAAAAACTACCA60.5655042412818096No Hit
CCTCCTATGAGAGATACGATTGCTACAAGGTCCCAGAATGGTGCTTAGAT50.47125353440150797No Hit
GATCTTCAAGTGAACATCTCTTGCCATCACCTAGCTGCCTGCACCTGCCC50.47125353440150797No Hit
GTGTAATAATTGGGTCTATTTCAAAATTAAGAAAATTAATAGCTTTAGGC50.47125353440150797No Hit
CTATATAAGGAAGGGGATGGGATTTTCCATTCTAAAAGTTTTGGAGAGGG50.47125353440150797No Hit
AGACATTATATATTGAGAGGCTATAAACTGTCAAGTGCTATGCAAATGTA50.47125353440150797No Hit
TATATGTACTTTGGAATATCTAACCTCCCTTGGTGATAAATTAAAGAGGA50.47125353440150797No Hit
GGGTGAGACCTCGTACAGGTGGGGGAGGGTGTTTTGAGACTTAGCTGAGA50.47125353440150797No Hit
GTTTCTAGCTAATTTCACAAAATTGAGACCATCCATGGCCTTTCGACACC50.47125353440150797No Hit
GGGTAACTTTTCAGCTCTAGTGTCATCATTCACTTAATTTACACTTTCAT40.3770028275212064No Hit
TAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGTTTTGTGGAC40.3770028275212064No Hit
GAAGAGTATGATGGAAATGTGATGAAAGAATTAAAATTGTTCACTTTAAA40.3770028275212064No Hit
GTTATTAGCATCCTAATCACTAGCTAGGCATAGTTTCCGCACCTGGCCGA30.2827521206409048No Hit
CTTCCAGACCATGGAGGAGAAGAAAGCATTCATGGGACCACTGAAGAAAG30.2827521206409048No Hit
CTCCAGAAGAGGAGAAGAGGAAACACAAGAAGAAACGCCTGGTGCAGAGC30.2827521206409048No Hit
GGGTAAAATCTTGCAAAGAGCTTTTATAATTTGTTTTACGGAATTGTATG30.2827521206409048No Hit
AAAGCAAGACTGTGGTTTCATTCCAATTTCCTGTATATCGGAATCATCAC30.2827521206409048No Hit
GTGCAAATACACTGTCCAGGATGAGAGCCACTCAGAGTGGGTGTCTTGTG30.2827521206409048No Hit
CCCCACTTCATATGTCGAAGTCTGTTTGGACAAAAATGCCAAAGGTGCTA30.2827521206409048No Hit
GATCCAAGTAAATCAGCACGACCAGTCAACATCAACATCATTATAAACCA30.2827521206409048No Hit
CCTCTGAGACCCACCTTGCTCATAAACAAAATGCCCATGTTGGTCCTCTG30.2827521206409048No Hit
GAGCAAGACTCTGCCTCACTAAAAAAAAAAAATAAAGTCAAAAATGGACA30.2827521206409048No Hit
ATCAACGCAGAGTACATGGGGGGAATTCGAGCTCGCATTTTGAAAATTCT30.2827521206409048No Hit
CTTCTCGAGAAGAGCAACCACAAGACACGTAATTGTCAGATTCACCAAGG30.2827521206409048No Hit
GATTTAGGGACTGGCAAGATAATCAACTTTATCAAATTTGATACAGGCAA30.2827521206409048No Hit
CTTCTAAATTTGTAATTTCTTATAACCAAAGCAGCTCTTCACATTTAAAA30.2827521206409048No Hit
ACGCTCTCCAGTGGAACAAGTTGCTGGTCCTCCTTGTTGGTGTTTATTAT20.1885014137606032No Hit
AGCTGGAGGTGGGTTCCATTTAGGCAGCACTGGAGCCCAGTTGGATGGGG20.1885014137606032No Hit
TACACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATA20.1885014137606032No Hit
GTATAAGAAAGCTCATGCTGCTATACGAGAGAATCCAGTCTATGAAAAGA20.1885014137606032No Hit
GCTTGAGCCCAGGAGTTTGAGATCAGTCTGGGCAACATAGGGAGATCCTA20.1885014137606032No Hit
GCAACCGAGGCCGGCAGGCCAAGGAGCGCTCCCGCCGAAGGCTCTCCCCA20.1885014137606032No Hit
CAAAGAGGAAGTTGATATAAGGTGTATTCATTTCTCTCTGCCTCAGGTTC20.1885014137606032No Hit
TTCCAGCACGGCTCAAACATTCCAGAGAAAACAAGATCTACATAGCAGGC20.1885014137606032No Hit
GGCGTGCAGTTTCCCGGCTCTCCGCGCGGCCGGGGAAGGTCAGCGCCGTA20.1885014137606032No Hit
GCAAGACACTGTCTGTTGCCCCAGGGCAGTCACCTCGCAGGAATGGTGCT20.1885014137606032No Hit
GTCTGGCTATACAACGCAGTAAGGTTCACACCCACCCAGGGTGGCAGTTA20.1885014137606032No Hit
CACGACCCTGCCGTCACACTGAGGCACCTTGTGTCCCTGAGCAGAGTTAC20.1885014137606032No Hit
GGTTAATGATAACAATGCATCGTAAAACCTTCAGAAGGAAAGGAGAATGT20.1885014137606032No Hit
GTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCT20.1885014137606032No Hit
GAGCCACTGCTGCCCCTGGCCTCACCGGCCTCAGGAGACGGTGGGGCCGC20.1885014137606032No Hit
ATTGTGACCAGACCCCGGGAGCGGCCCATGTAGAAAGAGAGAGACCTCAT20.1885014137606032No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG20.1885014137606032No Hit
AAGGAAATGACATTGAGCTTGTTTCAAATTCAGCGGCTTTGATTCAGCAA20.1885014137606032No Hit
GTCTTATATCTTTACCAATAAAACCACTGTCCAG20.1885014137606032No Hit
GTTTTAAGTTATTAGTTTTTAAAATCAGTACTTTTTAATGGAAACAACTT20.1885014137606032No Hit
GGATGCCGGAGAGCTGTGACTTCTCTGTGCCCTGGGCCCAAACTATGAAG20.1885014137606032No Hit
CCCCTCACTGCTGGGGAGTCCCTGCCACACTCAGTCCCCCACCACACTGA20.1885014137606032No Hit
ACCCCATCTCTACTTAAAATACAAAAATTAGCTGGGCATGGTGGTGTGTG20.1885014137606032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)