FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008161074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008161074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8830
Total Bases1.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGGGG951.0758776896942241No Hit
GGTATCAACGCAGAGTACATGGGGG820.928652321630804No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT690.7814269535673839No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC510.5775764439411099No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC510.5775764439411099No Hit
GTATCAACGCAGAGTACATGGGGGGG500.5662514156285391No Hit
TATCAACGCAGAGTACATGGGGGGG460.5209513023782559No Hit
GGTATCAACGCAGAGTACATGGGGGG350.39637599093997733No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC280.3171007927519819No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT260.2944507361268403No Hit
GTATCAACGCAGAGTACATGGGATG260.2944507361268403No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC250.28312570781426954No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT250.28312570781426954No Hit
GTATCAACGCAGAGTACATGGGGTG240.2718006795016987No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC230.26047565118912797No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG220.2491506228765572No Hit
TATCAACGCAGAGTACATGGGATTG220.2491506228765572No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG210.23782559456398644No Hit
CCACTAATACATGATTGGGTGGTTGGAGATGGTCTGAGAGATGGATTTTT200.22650056625141565No Hit
TCGTTGCACTTTATGATTTTGTAGCAAGTGGTGATAACACACTCAGCATC200.22650056625141565No Hit
TAATATGACAGGCCGCTAAAGACGTTCTACTCCGCCATATGAGATAATAT190.21517553793884486No Hit
GGTATCAACGCAGAGTACATGGGAG190.21517553793884486No Hit
GTGATAACACACTCAGCATCACTAAAGGTGAGGCTTCTATGTAGCATCCT180.20385050962627407No Hit
GCATGATGGAGGGGAACATGGCTCGGGGAACTATACCCAGCAAAGCCAGC180.20385050962627407No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG170.19252548131370328No Hit
GGAGAATGGCGTGAACCCAGGAGGCGGAGGTTGCAGTGAGCTGAGATCGC170.19252548131370328No Hit
GTATCAACGCAGAGTACATGGGGAG160.1812004530011325No Hit
GTACATGGGAAGGTAGTAGTTATGTTCCCTGTGGTCATTTATATTTTGGC160.1812004530011325No Hit
TCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCA160.1812004530011325No Hit
GTAGAGGCAGGCTGTGTTCACCAAAGTCAATCAGGGCTCAGCCAGAAAAA160.1812004530011325No Hit
GGGAAGAGCTGAACGGGGCAATGGAGCATACCAACAGCAACGAGAGTGAC150.16987542468856173No Hit
GTCAATAGCTAAAGGAATGTATTTACATTCAAAAGAGAATTTTAGGAACT150.16987542468856173No Hit
CAGCAAAATCAGCATACAAGGGACACACTTCAGTGTAATAAAAGCCATCT150.16987542468856173No Hit
TAATACTTCTGTGGACCGGGCGTGGTGGCTCACACCTGTAATCCCAGCAC150.16987542468856173No Hit
CATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCG150.16987542468856173No Hit
ACAGCAATCCATTCAGTGAAGAGGTTTCGCTAGGTTGACATAAAAATAGA150.16987542468856173No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG150.16987542468856173No Hit
ATGTGCACATGTACCCTAAAACTTAAAGTATAATAATAAAAAAAAATCTC140.15855039637599094No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA140.15855039637599094No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC140.15855039637599094No Hit
GTATCAACGCAGAGTACATGGGAGG140.15855039637599094No Hit
GGCCATTCTTTCAAGCAGTAGCTTTAGAAATTCACTTCGATTTGGAAGAA140.15855039637599094No Hit
GTATCAACGCAGAGTACATGGGGAAG140.15855039637599094No Hit
GTCTGGGGATGTGTGGTTATAGTTCTGTGGGAGAAATAATTTTGTCAGTG130.14722536806342015No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT130.14722536806342015No Hit
TCCCAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAG130.14722536806342015No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG130.14722536806342015No Hit
CACCTGCCCTGTGAAGTTGAGGGCTGTGTGGCCTGATTAAAGAAGCATGC130.14722536806342015No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCTCTTGCTTCAACAATAACG130.14722536806342015No Hit
AACTTGGGCCGTTCAGAATAGTGTATTTCCAGTGCAGCTCTGGAAAGAGT130.14722536806342015No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG130.14722536806342015No Hit
AGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGC130.14722536806342015No Hit
ATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATA130.14722536806342015No Hit
ATTTTATTCTTTTAAAAAGAACACTCCGGCCGGGCGCGGTGGCTCACGCC130.14722536806342015No Hit
GGTATCAACGCAGAGTACATGGGGGGG120.13590033975084936No Hit
CCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGG120.13590033975084936No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG120.13590033975084936No Hit
CCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCG120.13590033975084936No Hit
ATCGAGACCATCCCGGCTAAAACGGTGAAACCCCGTCTCTACTAAAAATA120.13590033975084936No Hit
GTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACC120.13590033975084936No Hit
GTACATGGGAGGAGAATGGGTAGGCCTTTGTAAAATTGACAGAGAGGGGA120.13590033975084936No Hit
CTGTGAATAGGCAGCGTGGGAGACAGGCTTTTCAAAATTTTTTTTCTTCT120.13590033975084936No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA120.13590033975084936No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC120.13590033975084936No Hit
CTAGATGCAGAGAAAACAGCTCCTTGGTGAATTGATAAGTAAAGGCAGAA120.13590033975084936No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC120.13590033975084936No Hit
AATATAGATAGAATGGCAGTGTGACATAGGGGCGCTGTGGTAGAGGGATG120.13590033975084936No Hit
GGTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTG120.13590033975084936No Hit
GCGTCCCCACTTGGTTGAAGTTGACATCTGACGACGTGAAGGAGCAGATT120.13590033975084936No Hit
AGCATAACCCTGAGATTCTTACTACTGATGAGAACATTATCTGCATATGC110.1245753114382786No Hit
GATCACAAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATTGTGAAACCC110.1245753114382786No Hit
ACAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCCTTGTCATA110.1245753114382786No Hit
CTCAAAGAGATTCGGAAATTTGCCATGAAGGAGATGGGAACTCCAGATGT110.1245753114382786No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA110.1245753114382786No Hit
CATATTAATGTTGAGAAGTTCTGCTTAATTTTAAAATGGTTTCTATAAAG110.1245753114382786No Hit
CACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCTTTTGCC110.1245753114382786No Hit
GTGTGGTGTCATGAGTATGTTAAAATTACTGCTATCTTTTTGTGTTCTTT110.1245753114382786No Hit
ATTCACCACTTAATAAGGAAATAAACTTATTATCAGGAAAGTATGTTGTG110.1245753114382786No Hit
GCAAAAAAGGTGCTAGCTGGTCTGGTACTTAGTACTTTAGAAAAGTTGCC110.1245753114382786No Hit
GGCCCTTTGACCTCTGGTCTGAACACTGACCTATAATCTAGTGCTCCATA110.1245753114382786No Hit
GTCTAGCACTGTAGTTGGTGCATGTACTCGACTGCCGCTGCATCTACTAT110.1245753114382786No Hit
GGTATCAACGCAGAGTACATGGGGTG110.1245753114382786No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT110.1245753114382786No Hit
CTTATGTTATTAACCATTTCCTTAAATTCTTCTGGGTCTGCTGACAAAGC110.1245753114382786No Hit
CCATGAATAAGCTCTGGCTGGTGTTCACCATGAATAAGCCCAGGTTCCTC110.1245753114382786No Hit
CTACAGCACGGCCTGTGGCCTGTTCAAGGCAGAACCACGACCCTTGACTC110.1245753114382786No Hit
GAATTTAATAGTGACCTATTCAATAAGGCAATCATCTTGGTAGCAAACTT110.1245753114382786No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTTTTAACTTGTCATATCGC110.1245753114382786No Hit
CTTAATAATACTTCTGTGGACCGGGCGTGGTGGCTCACACCTGTAATCCC110.1245753114382786No Hit
GGGAGGGATAGCATTGGGAGATATACCTAATGCTAGATGACGAGTTAGTG110.1245753114382786No Hit
CACCTGTAATCCCCACACTTTGGGAGGCCGAGGCGGGCGGATCACCTGAG110.1245753114382786No Hit
CATTTGGATGTCCAGGAGGACAGGGAAGCTGTTCTGTTTTGTTCATTCCT100.11325028312570783No Hit
GTTATACGGAAGATGATGAACCGCTTCATGCCACTGAATACGTTGAAAAA100.11325028312570783No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT100.11325028312570783No Hit
TGCCATGAGTGTGCACACATGCATGTGTCTGTGTATGTGTGAATGTGAGA100.11325028312570783No Hit
TGGTGGAACTCCATCTTTAGTAAAAATGCAAAAAAATTAGCCAGGCATGG100.11325028312570783No Hit
GTCTTATGATAACTGTGACCCTGAGCCTCAGTGTTTTCCTTTTAATCTGT100.11325028312570783No Hit
GAGTACATGGGAAGATGACTGTTTTAAAGCTATGTGGGCTGGGGGCAGTG100.11325028312570783No Hit
CTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAGAA100.11325028312570783No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT100.11325028312570783No Hit
CTTTAGAAATTCACTTCGATTTGGAAGAAATTAGTTTCTGTCACTTGACT100.11325028312570783No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA100.11325028312570783No Hit
GTATCAACGCAGAGTACATGGGATTG100.11325028312570783No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC100.11325028312570783No Hit
GTATCAACGCAGAGTACATGGGAGTCGCTATCGGAGTAGCCGCGCTGGTG100.11325028312570783No Hit
AGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGGCAATCG100.11325028312570783No Hit
AATAAGGCCTAGCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTA100.11325028312570783No Hit
CCTCAAGCCAGGGCTCCTCCTCCTGTCGTGGGCTCATTGTGACCACTGGC100.11325028312570783No Hit
GGAAAACACCAAACTCTTCTGCTCTGTCTTCTGAAGAGGCCATTCTTTCA100.11325028312570783No Hit
ATACAGAGGCTGTTGTCTACTTAACTCGTTCAGAAAAATGCAGCTTTTGA100.11325028312570783No Hit
TATCAACGCAGAGTACATGGGACTGTGACCCCTGTTCCCATGCTGACCTG100.11325028312570783No Hit
GAGAGAAGACACAAATTAACAGTATTAGAAATGAGAGAGTTGGCATAACA100.11325028312570783No Hit
GATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGC100.11325028312570783No Hit
GGTATCAACGCAGAGTACATGGGATG100.11325028312570783No Hit
TAGCAGGGCTCCCTTCATTGCATCTGAGGACTTGTTTTCTTTTTCTTTAT100.11325028312570783No Hit
ATATTAGTGAAATTAGTATTAACATTTTCTACTGTGGCAACTTACAATAA100.11325028312570783No Hit
CCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGTCTCTGGGACTA100.11325028312570783No Hit
GAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTAT100.11325028312570783No Hit
CTTTGGAAACTGAGGACGCGTGGATTCTACTCAAGCCTCCAAGTAGTGGC100.11325028312570783No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA100.11325028312570783No Hit
CCAACAAGATGGCCCCAGCGCTTCGCAAGGTCTACGACCAGATGCCGGAG100.11325028312570783No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA100.11325028312570783No Hit
GCACTGATATGAGCCAGTGCTGCTGCTCTCTACGGAGTGTGCTCGGCTAT100.11325028312570783No Hit
ATCTTAGACAATGGGGGCAACCCTGATCTAGCCATGCCGCGTGAGTGATG90.10192525481313704No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT90.10192525481313704No Hit
GTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATT90.10192525481313704No Hit
GATCATGAGGTCAGGGGTTCGAGACCAGCCTGGCCAATATGGTGAAACCC90.10192525481313704No Hit
GTACATGGGAGAGAAGGCAGTCTCTCTCTCAAAACACACAAAAAGCACTC90.10192525481313704No Hit
GAATAGCAATGATGCTTTCTTTATAGGGCTGAGGTGACGATTAAGAGTTA90.10192525481313704No Hit
CACGTAGTGAGCATCCTGTGCTCGCATATACCGCTGCTGCACTGATATGA90.10192525481313704No Hit
GGAGAGAGACTAAAGAAATGGAAGGAAGACCTCTAGAAATTACCCATAAT90.10192525481313704No Hit
GTTTAGAAGTAGACAGCAGAAGAATCCAGCAAAAATAGTAGACACCATGA90.10192525481313704No Hit
ACATAGCTGTTCCCTAACTTGGTGGATGGCTCCAGTTTGCTTTTAACGAA90.10192525481313704No Hit
CCATAGGGCATGATTAACAAAGCAACCTTTCCCTTCCCCCGAGTGATTTT90.10192525481313704No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC90.10192525481313704No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC90.10192525481313704No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA90.10192525481313704No Hit
GAATCACTTGAACCTGGGAGGCAGAAGTTGCAGTGAGCTGAGATTGCGCC90.10192525481313704No Hit
GCTCCAACAAAACTGACAGCAAAAAAAGACAGAGAGACAAATTACCAATA90.10192525481313704No Hit
AATTGGCGCACAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACAC90.10192525481313704No Hit
GCCTTACTAGTTGCATTGAAAAAGATGTAATGTAGTCCAGGCGCAGTGGC90.10192525481313704No Hit
GTATCAACGCAGAGTACATGGAGAG90.10192525481313704No Hit
CTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG90.10192525481313704No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT90.10192525481313704No Hit
ATATTTATACTGTCTGGGGATGTGTGGTTATAGTTCTGTGGGAGAAATAA90.10192525481313704No Hit
GTATCAACGCAGAGTACATGGGGTTTAAGGTTTCCTCAAACTGTGATTTT90.10192525481313704No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA90.10192525481313704No Hit
GAATTAAAGAACATGCGTAAAACGACCTCGTTGTAATTTGGGTCGGCCCG90.10192525481313704No Hit
GATTATCCTGGATTGTCTCCGTGGGCATTAAATCCTGGCACATATATCCT90.10192525481313704No Hit
GTGTTAGATATTGAGGCTGCTTCGTGGGCCGAAGTGTCTTCCGATGCAGT90.10192525481313704No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG90.10192525481313704No Hit
GGCGGGCAGGTCACCTGAGGTCAGGAGTTTGAGACCAACCTGGCCAACAT90.10192525481313704No Hit
GTACAAACCTTGGAAAAAAAACACATTAGTCTATGGGACCTTCCAGTTTT90.10192525481313704No Hit
TGTCATAGTAGCCATCATAATGTTTGACACAATGCATCACTCATATGTTT90.10192525481313704No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG90.10192525481313704No Hit
CAACAATCTAAATTATTTGGGAAAAGATGAAGCTGAATCAAATATAGAGA90.10192525481313704No Hit
CTCTTTCATTTGCTGCCCAGGATGGTGATATTAACTAGTTTTTTGAACTT90.10192525481313704No Hit
TATCAACGCAGAGTACATGGGGAAGG90.10192525481313704No Hit
CCATTGTGGGTATTCCTATTTGGTACACAAATTTCTTTCACATGGTAAAT90.10192525481313704No Hit
GTACTCGTAAAACAAGGATCATCGATGTTGTCTACAATGCATCTAATAAC90.10192525481313704No Hit
ATAAAAATAATCTTCAAAAAATAAAAACACTGCAAACAAACACGAAGAAA90.10192525481313704No Hit
GAGGTGAGCAGATCACCTGTAGTCAAAAGTTTGAGGCCAGCCTGACCAAC90.10192525481313704No Hit
GGGTATCACCGCCCTACACATCAAACTCCGGGCCACAGGAGGAAATAGGA90.10192525481313704No Hit
TTATTACTCTGTTCTAAAGTTAAACTCCAAAAGAGGGAACAATCCAGGAA90.10192525481313704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)