FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008161289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008161289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14063
Total Bases2.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3032.1545900590201237No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2311.642608262817322No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1190.8461921353907417No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1130.8035269857071748No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1120.7964161274265804No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1100.7821944108653914No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA880.6257555286923132No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG790.561757804166963No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT680.4835383630804238No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG620.440873213396857No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA610.43376235511626254No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT590.4195406385550736No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC560.3982080637132902No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA540.3839863471521013No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC510.36265377231031787No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG480.34132119746853445No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT480.34132119746853445No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT480.34132119746853445No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA470.33421033918794No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC460.32709948090734553No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT450.3199886226267511No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC390.27732347294318427No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT380.2702126146625898No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT370.26310175638199534No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT360.25599089810140085No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC360.25599089810140085No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT340.2417691815402119No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG310.2204366066984285No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA300.21332574841783403No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG300.21332574841783403No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC300.21332574841783403No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG300.21332574841783403No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC290.20621489013723956No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT280.1991040318566451No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG280.1991040318566451No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG270.19199317357605064No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG270.19199317357605064No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG260.18488231529545618No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT260.18488231529545618No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC260.18488231529545618No Hit
AACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTA260.18488231529545618No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT250.1777714570148617No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT240.17066059873426723No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC240.17066059873426723No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA240.17066059873426723No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA230.16354974045367277No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT220.1564388821730783No Hit
GGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAA220.1564388821730783No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT220.1564388821730783No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG220.1564388821730783No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG220.1564388821730783No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG210.14932802389248384No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT210.14932802389248384No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC200.14221716561188935No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA200.14221716561188935No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA200.14221716561188935No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA200.14221716561188935No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT200.14221716561188935No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA190.1351063073312949No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT190.1351063073312949No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC190.1351063073312949No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG190.1351063073312949No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG190.1351063073312949No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA190.1351063073312949No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT190.1351063073312949No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG190.1351063073312949No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC190.1351063073312949No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT180.12799544905070043No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA180.12799544905070043No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT180.12799544905070043No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT180.12799544905070043No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG180.12799544905070043No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG180.12799544905070043No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT180.12799544905070043No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT170.12088459077010595No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC170.12088459077010595No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA170.12088459077010595No Hit
GGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC170.12088459077010595No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT170.12088459077010595No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC170.12088459077010595No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA170.12088459077010595No Hit
GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT170.12088459077010595No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG160.11377373248951149No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA160.11377373248951149No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT160.11377373248951149No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC160.11377373248951149No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG160.11377373248951149No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA160.11377373248951149No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT150.10666287420891701No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG150.10666287420891701No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC150.10666287420891701No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT150.10666287420891701No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT150.10666287420891701No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG150.10666287420891701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)