FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008162364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008162364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81629
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5770.7068566318342745No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5260.6443788359529089No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4300.526773573117397No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2720.33321491136728365No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2680.3283146920824707No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC2420.2964632667311862No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG2320.28421271851915375No Hit
GGATTCATCTTTCTTTTCACCGTAGGTGGCCTGACTGGCATTGTATTAGC2030.24868612870425955No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1930.23643558049222702No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1930.23643558049222702No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1890.23153536120741405No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG1860.22786019674380428No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG1770.21683470335297503No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1750.21438459371056853No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1730.21193448406816207No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1700.2082593196045523No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT1690.20703426478334905No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1690.20703426478334905No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1650.20213404549853606No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1620.19845888103492632No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1550.18988349728650358No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA1510.18498327800169057No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA1490.1825331683592841No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC1470.18008305871687758No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT1420.17395778461086134No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1410.1727327297896581No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1400.17150767496845484No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1360.16660745568364183No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1320.16170723639882886No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1290.1580320719352191No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1290.1580320719352191No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA1280.15680701711401585No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1280.15680701711401585No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT1280.15680701711401585No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1270.15558196229281263No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA1260.15435690747160935No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA1240.15190679782920286No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC1240.15190679782920286No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA1230.1506817430079996No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT1220.14945668818679636No Hit
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT1210.1482316333655931No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC1180.14455646890198337No Hit
CTACTCCCACTAATAGCTTTTTGATGACTTCTAGCAAGCCTCGCTAACCT1170.14333141408078012No Hit
TCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTCAACGATTAAAGT1160.14210635925957688No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT1110.13598108515356064No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1080.13230592068995087No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG1080.13230592068995087No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1050.12863075622634113No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC1050.12863075622634113No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1050.12863075622634113No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA1040.12740570140513788No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA1040.12740570140513788No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1040.12740570140513788No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC1030.12618064658393463No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG1030.12618064658393463No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1020.12495559176273138No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG1010.12373053694152814No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA1010.12373053694152814No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG1000.12250548212032487No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT1000.12250548212032487No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG990.12128042729912163No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC990.12128042729912163No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA980.12005537247791839No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG980.12005537247791839No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT980.12005537247791839No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA970.11883031765671515No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT970.11883031765671515No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG970.11883031765671515No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG960.1176052628355119No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT950.11638020801430864No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT950.11638020801430864No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG950.11638020801430864No Hit
GACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATAC950.11638020801430864No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG940.11515515319310539No Hit
AACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGA940.11515515319310539No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC920.11270504355069888No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC920.11270504355069888No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT920.11270504355069888No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT920.11270504355069888No Hit
ATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTC920.11270504355069888No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC920.11270504355069888No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC910.11147998872949563No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT910.11147998872949563No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT900.1102549339082924No Hit
CCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAG880.1078048242658859No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG870.10657976944468264No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACAT870.10657976944468264No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT860.1053547146234794No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG860.1053547146234794No Hit
TTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCA850.10412965980227615No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT830.10167955015986967No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG820.1004544953386664No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA820.1004544953386664No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCCCACTTCCA820.1004544953386664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)