FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008162411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008162411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23009
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4401.91229518883915No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2811.221261245599548No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2541.103915859011691No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2260.9822243469946543No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1520.6606110652353426No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1500.6519188143769828No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1410.6128036855143639No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1390.6041114346560043No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1350.5867269329392846No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA1290.5606501803642053No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1110.4824199226389674No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1080.4693815463514277No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1060.4606892954930679No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA960.4172280412012691No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG930.4041896649137294No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG900.3911512886261898No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA880.38245903776782997No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC840.36507453605111045No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC820.35638228519275067No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT790.34334390890521105No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC790.34334390890521105No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG740.3216132817593116No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG730.3172671563301317No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT730.3172671563301317No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT700.304228780042592No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC700.304228780042592No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG640.2781520274675127No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT620.26945977660915293No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA620.26945977660915293No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC600.26076752575079315No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT590.25642140032161326No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT580.25207527489243337No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG580.25207527489243337No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT570.2477291494632535No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT570.2477291494632535No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC560.2433830240340736No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG560.2433830240340736No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT560.2433830240340736No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG560.2433830240340736No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC560.2433830240340736No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG550.23903689860489374No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT550.23903689860489374No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC540.23469077317571385No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC530.23034464774653396No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC530.23034464774653396No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC530.23034464774653396No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG520.22599852231735407No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA500.2173062714589943No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT490.21296014602981442No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT480.20861402060063455No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT480.20861402060063455No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA480.20861402060063455No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG480.20861402060063455No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG470.20426789517145466No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA460.19992176974227477No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG460.19992176974227477No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT450.1955756443130949No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT440.19122951888391498No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA430.1868833934547351No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA420.18253726802555523No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC420.18253726802555523No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT420.18253726802555523No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT410.17819114259637533No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC410.17819114259637533No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT410.17819114259637533No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT400.17384501716719544No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT390.16949889173801555No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA390.16949889173801555No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT380.16515276630883566No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC380.16515276630883566No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC380.16515276630883566No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT380.16515276630883566No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG380.16515276630883566No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT370.1608066408796558No Hit
TCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCG370.1608066408796558No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG370.1608066408796558No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG370.1608066408796558No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA360.1564605154504759No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG360.1564605154504759No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC350.152114390021296No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA350.152114390021296No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG350.152114390021296No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC340.14776826459211612No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC340.14776826459211612No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC340.14776826459211612No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC340.14776826459211612No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT330.14342213916293625No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG330.14342213916293625No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT320.13907601373375636No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG320.13907601373375636No Hit
AAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCA320.13907601373375636No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA320.13907601373375636No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG320.13907601373375636No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA320.13907601373375636No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG320.13907601373375636No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC320.13907601373375636No Hit
GTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGA310.13472988830457647No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA310.13472988830457647No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG310.13472988830457647No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT310.13472988830457647No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA300.13038376287539657No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA300.13038376287539657No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT300.13038376287539657No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG290.12603763744621668No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT290.12603763744621668No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT280.1216915120170368No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG280.1216915120170368No Hit
TCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTA280.1216915120170368No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA280.1216915120170368No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA280.1216915120170368No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG280.1216915120170368No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC280.1216915120170368No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT280.1216915120170368No Hit
CCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCT270.11734538658785693No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA270.11734538658785693No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG270.11734538658785693No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG270.11734538658785693No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG270.11734538658785693No Hit
TCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAAAAG270.11734538658785693No Hit
GAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTA270.11734538658785693No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC270.11734538658785693No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC260.11299926115867703No Hit
TGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAAC260.11299926115867703No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG260.11299926115867703No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC260.11299926115867703No Hit
TACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGA260.11299926115867703No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC260.11299926115867703No Hit
GTACATGATTCGTCAGTGTGGATCAATTACAGTTAGGCCGCTGACACATT260.11299926115867703No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT260.11299926115867703No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT250.10865313572949716No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG250.10865313572949716No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG250.10865313572949716No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA250.10865313572949716No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA250.10865313572949716No Hit
ACATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAA250.10865313572949716No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC240.10430701030031728No Hit
GCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGG240.10430701030031728No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA240.10430701030031728No Hit
CTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTT240.10430701030031728No Hit
TCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGC240.10430701030031728No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC240.10430701030031728No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT240.10430701030031728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)