FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008162429

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008162429
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455887
Total Bases240 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACTAGAGCTGCA79920.5489437023615157No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC46630.3202858463603288No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC46570.319873726463661No Hit
GTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTG44920.30854042930529635No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43770.3006414646191634No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT41640.28601120828745635No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT41010.28168394937244445No Hit
GGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACC40850.2805849629813303No Hit
AGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTC39880.27392235798520076No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATA38010.2610779545390542No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCT37100.2548274694395925No Hit
CTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACT36490.2506375838234698No Hit
CCCCAAGGCATCAAGCCCTTCTCCCTGCACTCAATAAACCCTCAATAAAT34940.23999115315955152No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC33730.23168006857675083No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA33480.22996290234063493No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCA32850.225635643425623No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC31330.21519527271003863No Hit
CAATAAACCCTCAATAAATATTCTCATTGTCAATCAG30800.211554880289473No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG30200.207433681322795No Hit
GCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTC29730.20420540879889718No Hit
CCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAG29530.2028316758100045No Hit
TCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGGCATCAAGCCCTTCTCC27510.1889569726221884No Hit
GTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA27510.1889569726221884No Hit
GTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCA26680.18325598071828375No Hit
ATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTC26250.1803024547921645No Hit
GACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCACCCCAAGG25950.17824185530882547No Hit
CCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGAC25320.17391459639381354No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA24710.16972471077769086No Hit
AGCTACAGCTGCCAGGTCACGCATGAAGGGAGCACCGTGGAGAAGACAGT24040.1651227052649004No Hit
GTTCTCATCCCTCACCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCA23640.162375239287115No Hit
CTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGA23620.16223786598822573No Hit
CCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCATCCCT22530.15475102119876064No Hit
GGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAA20820.14300560414372818No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20720.14231873764928185No Hit
CCCCCCACCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCT19760.13572481930259697No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA19710.1353813860553738No Hit
GCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAA19350.132908666675367No Hit
CGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGCCAGGTCACGCAT18910.12988645409980307No Hit
GGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCG18830.129336960904246No Hit
CAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAG18760.12885615435813358No Hit
CTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCG18090.12425414884534307No Hit
CCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCC17930.12315516245422894No Hit
TCCTATGACAGCAGCCTGAGTGGTTGGTTGTTCGGCGGAGGGACCAAGCT17670.12136930956866845No Hit
ACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGGAGACTAG17440.11978951663144187No Hit
GCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAG16970.11656124410754407No Hit
CCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCG16580.11388246477920332No Hit
GGCCCCTACAGAATGTTCATAGGTTCTCATCCCTCACCCCCCACCACGGG16280.11182186529586431No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGC16090.11051681895641627No Hit
CCACGGGAGACTAGAGCTGCAGGATCCCAGGGGAGGGGTCTCTCCTCCCA16030.11010469905974846No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAA15800.10852490612252186No Hit
CAATAAACCCTCAATAAATATTCTCATTGTC15560.10687642653585065No Hit
CCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTG15290.10502188700084554No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG15280.10495320035140089No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC15250.104747140403067No Hit
CAATAAACCCTCAATAAATATTCTCATTGTCAATC15230.10460976710417773No Hit
GTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCT14660.10069462808583358No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)