FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008162463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008162463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781206
Total Bases463.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG277890.9991708632873653No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT231760.8333075651354124No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC186090.6690982257337285No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC152860.5496176838393129No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA139940.5031630163317641No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG136980.4925201513300346No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC115600.41564702506754264No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA112230.40352997944057367No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC105030.3776419294363668No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT102120.36717884255966654No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA92570.3328412206790867No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC91460.3288501463034381No Hit
CTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATA82880.2980002200484251No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC80360.28893940254695266No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA79120.28448090504622814No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT78740.2831145912960061No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC78300.281532543795749No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG75680.2721121700442182No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC75000.2696671875438209No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG72960.26233224004262895No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC72350.2601389469172726No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA72330.26006703566726086No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG72150.2594198344171557No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT70990.255248981916478No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC67300.24198135628932196No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT66350.2385655719137669No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA65390.23511383191320598No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC64260.23105084628754577No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT63570.22856990816214262No Hit
ACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG61730.22195407316106755No Hit
CACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTG60570.21778322066038977No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC58110.20893813690895247No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA57270.20591786440846163No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA56910.20462346190825131No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG56610.20354479315807603No Hit
CCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCT56510.2031852369080176No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG55840.20077621003262613No Hit
ATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTT55330.19894247315732816No Hit
ATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTAT55260.19869078378228727No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC54660.1965334462819367No Hit
GGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGG50760.18251075252965798No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC49640.1784837225290036No Hit
CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT48410.17406118065328494No Hit
CCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAAT46420.16690601127712223No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA45630.16406551690166066No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC45210.16255538065141523No Hit
GGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATC43520.1564788800254278No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA43050.1547889656501532No Hit
CAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTC40140.14432587877345296No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT39870.1433550768982952No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC39470.1419168518980615No Hit
GCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAA39400.14166516252302058No Hit
CCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTG39130.14069436064786284No Hit
CCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCT38990.14019098189778104No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA37840.1360560850221091No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC37740.13569652877205068No Hit
GTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTG37620.13526506127198057No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCA37230.1338627918967527No Hit
GTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA36480.13116612002131448No Hit
TTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAG35880.12900878252096393No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG35170.1264559331455491No Hit
CCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCC34990.12580873189544392No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACA33750.12135023439471941No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT32840.11807827251918773No Hit
GTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCC32670.11746702689408839No Hit
CCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCC32180.1157052012688021No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT31870.11459057689362097No Hit
AGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGG31820.11441079876859175No Hit
GCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC31610.11365573064346907No Hit
GCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGG31050.11164221564314185No Hit
GGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCA30910.11113883689306005No Hit
TCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGT30550.10984443439284973No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC30500.1096646562678205No Hit
GCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCA30170.10847812064262771No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)