FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008162583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008162583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16124
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2961.8357727611014636No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2171.3458198958074918No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1981.2279831307367899No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1100.6822128504093277No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC1080.6698089804018854No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1070.6636070453981642No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA970.6015876953609526No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC800.49615480029769293No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG750.4651451252790871No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG730.45274125527164477No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA700.4341354502604812No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA700.4341354502604812No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA670.4155296452493178No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA660.40932771024559667No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT640.3969238402381543No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA640.3969238402381543No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT620.384519970230712No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT620.384519970230712No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG600.37211610022326963No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG600.37211610022326963No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT560.347308360208385No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT550.34110642520466383No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT530.3287025551972215No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG510.3162986851897792No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC480.2976928801786157No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT480.2976928801786157No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT460.2852890101711734No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC460.2852890101711734No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG440.27288514016373105No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG430.26668320516000993No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT420.2604812701562888No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT420.2604812701562888No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG400.24807740014884647No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG400.24807740014884647No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC400.24807740014884647No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT390.24187546514512526No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA380.23567353014140413No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC360.2232696601339618No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA360.2232696601339618No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA360.2232696601339618No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT350.2170677251302406No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC350.2170677251302406No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA340.2108657901265195No Hit
GGAATAAGCTGACGGTGACAAGGTTTCCCCCTAATCGAGACGCTGCAATA340.2108657901265195No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT330.20466385512279833No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC330.20466385512279833No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA320.19846192011907715No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC320.19846192011907715No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG320.19846192011907715No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA320.19846192011907715No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA310.192259985115356No Hit
ATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAG310.192259985115356No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC310.192259985115356No Hit
TTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGT310.192259985115356No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC310.192259985115356No Hit
GACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCA300.18605805011163482No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG300.18605805011163482No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC290.1798561151079137No Hit
ATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTTCTTAAAGGACT290.1798561151079137No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT280.1736541801041925No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC270.16745224510047135No Hit
GGGTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCC270.16745224510047135No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT260.1612503100967502No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT260.1612503100967502No Hit
AAGTAGGGCCATATGCCTCGGGAATAAGCTGACGGTGACAAGGTTTCCCC260.1612503100967502No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC260.1612503100967502No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA260.1612503100967502No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG260.1612503100967502No Hit
GATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGC260.1612503100967502No Hit
CCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTAC260.1612503100967502No Hit
TTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATA250.15504837509302902No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC250.15504837509302902No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG250.15504837509302902No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC250.15504837509302902No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC250.15504837509302902No Hit
TCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATG250.15504837509302902No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT250.15504837509302902No Hit
AGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATACGCTCTAAGCTCGG240.14884644008930786No Hit
GTGTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGT240.14884644008930786No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG240.14884644008930786No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC240.14884644008930786No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC240.14884644008930786No Hit
CTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCG240.14884644008930786No Hit
ATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTA230.1426445050855867No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG230.1426445050855867No Hit
GAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTAAAGACAAA230.1426445050855867No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA230.1426445050855867No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA230.1426445050855867No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC220.13644257008186553No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT220.13644257008186553No Hit
CCATATCAGAAATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCA220.13644257008186553No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC220.13644257008186553No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC220.13644257008186553No Hit
ATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTACTACTCC220.13644257008186553No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC220.13644257008186553No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA210.1302406350781444No Hit
ATACATAAGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGA210.1302406350781444No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG210.1302406350781444No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT210.1302406350781444No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC210.1302406350781444No Hit
AACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTT210.1302406350781444No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG210.1302406350781444No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT200.12403870007442323No Hit
CCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGG200.12403870007442323No Hit
TACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACA200.12403870007442323No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG200.12403870007442323No Hit
TCCTTCCGCAGTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGAC200.12403870007442323No Hit
TATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATG200.12403870007442323No Hit
TTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTG200.12403870007442323No Hit
TCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGT200.12403870007442323No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG200.12403870007442323No Hit
TTATCAGGTAGAGAACACTCGTGTAGGCCAAGTTGCAAACTATGATAAAC200.12403870007442323No Hit
GGACTGAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAA200.12403870007442323No Hit
GATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGT200.12403870007442323No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC190.11783676507070207No Hit
GCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT190.11783676507070207No Hit
CCCTACTCTTGCTGGTAAAATCACTGGCATGTTGTTGGAGATTGATAATT190.11783676507070207No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT190.11783676507070207No Hit
ATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTAC190.11783676507070207No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA190.11783676507070207No Hit
TCCTAGCACTTTGGGAAGCCCAGATCACTTGAGGCCAGGAGTTTGAGACC180.1116348300669809No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA180.1116348300669809No Hit
CCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGA180.1116348300669809No Hit
AAGGTGTACTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCA180.1116348300669809No Hit
CTAGTGTGGAGACGAATTGCCAGAATTATTAACTGCGCAGTTAGGGCAGC180.1116348300669809No Hit
CACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATC180.1116348300669809No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG180.1116348300669809No Hit
TTCGCCATGTTGCCCAGGCTAGTCTTGAACTCCTGGGCTCAAGCAATCCT180.1116348300669809No Hit
GGTAAAGATTTGATAGATGCATATATAAAAGTGGAGGTTTTAGAAGAACA180.1116348300669809No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG180.1116348300669809No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT170.10543289506325974No Hit
CAACATGAACGACTGCTACTCCAAGCTCAAGGAGCTGGTGCCCAGCATCC170.10543289506325974No Hit
TCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATC170.10543289506325974No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA170.10543289506325974No Hit
ATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTATGT170.10543289506325974No Hit
ATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGT170.10543289506325974No Hit
ACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGA170.10543289506325974No Hit
TACTTTAGGTGGGCCTTGGTTTCCTTCCGCAGTCAAAACCGCGCAATTAT170.10543289506325974No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT170.10543289506325974No Hit
GTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGA170.10543289506325974No Hit
GAATATTCGGTGGCAGTATGGGATTTCTAAAAATAATGTTAAGAATTTTT170.10543289506325974No Hit
CTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGC170.10543289506325974No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT170.10543289506325974No Hit
GTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGA170.10543289506325974No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT170.10543289506325974No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT170.10543289506325974No Hit
GTACATGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAG170.10543289506325974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)