FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008162813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008162813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261200
Total Bases46.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT7260.277947932618683No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4890.18721286370597245No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4270.16347626339969373No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4190.1604134762633997No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT3740.14318529862174578No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3570.13667687595712097No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG3320.12710566615620214No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT3210.12289433384379785No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT3160.12098009188361408No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG2800.10719754977029097No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA2730.1045176110260337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)