FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294732
Total Bases51.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT7300.24768264050052252No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA7010.23784319313817298No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG6640.22528941546896844No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT6250.2120570552230501No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG5780.19611036467027673No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG5580.18932453890313913No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT5260.17846721767571896No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT5160.17507430479215016No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA4650.15777044908594925No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4630.1570918665092355No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGA4360.14793100172359974No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4000.13571651534275206No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA3520.11943053350162182No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA3430.11637691190640989No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3410.11569832932969612No Hit
GTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCGATCTTTTAAAAA3350.11366258159955485No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA3330.11298399902284109No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA3170.10755533840913101No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC3000.10178738650706404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)