FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39755
Total Bases4.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACATGGGGG4761.1973336687209155No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3320.835115079864168No Hit
GTATCAACGCAGAGTACATGGGGGG2820.7093447365111307No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2800.7043139227770092No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2330.5860898000251541No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1280.3219720789837756No Hit
GTATCAACGCAGAGTACATGGGGTG1240.31191045151553265No Hit
TATCAACGCAGAGTACATGGGGGGG1140.28675638284492516No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1090.27417934850962145No Hit
GTATCAACGCAGAGTACATGGGATG1070.26914853477549994No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1040.2616023141743177No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT970.24399446610489248No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA950.23896365237077097No Hit
GTATCAACGCAGAGTACATGGGGAG890.22387121116840647No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC860.21632499056722423No Hit
GGTATCAACGCAGAGTACATGGGGGG840.21129417683310275No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT830.208778769966042No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA810.20374795623192052No Hit
TATCAACGCAGAGTACATGGGATGG740.1861401081624953No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT740.1861401081624953No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA740.1861401081624953No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG740.1861401081624953No Hit
GTATCAACGCAGAGTACATGGGAGG710.17859388756131303No Hit
GTATCAACGCAGAGTACATGGGGGGG690.17356307382719155No Hit
TATCAACGCAGAGTACATGGGAACAC650.16350144635894856No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT630.15847063262482708No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC630.15847063262482708No Hit
GTATCAACGCAGAGTACATGGGATGG610.15343981889070557No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG610.15343981889070557No Hit
TATCAACGCAGAGTACATGGGATTCCC600.15092441202364482No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC590.1484090051565841No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA580.14589359828952334No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA570.14337819142246258No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT560.14086278455540183No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC550.1383473776883411No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG540.13583197082128035No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA540.13583197082128035No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG530.1333165639542196No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG520.13080115708715884No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT520.13080115708715884No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA510.1282857502200981No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT480.12073952961891588No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC470.11822412275185512No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC470.11822412275185512No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC460.11570871588479437No Hit
TATCAACGCAGAGTACATGGGATTG460.11570871588479437No Hit
GGTATCAACGCAGAGTACATGGGATG460.11570871588479437No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT450.11319330901773363No Hit
GGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGG450.11319330901773363No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT440.11067790215067287No Hit
TATCAACGCAGAGTACATGGGGAAG440.11067790215067287No Hit
GTATCAACGCAGAGTACATGGAGAG430.10816249528361212No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC430.10816249528361212No Hit
GTATCAACGCAGAGTACATGGGGCG430.10816249528361212No Hit
GGTATCAACGCAGAGTACATGGGGTG420.10564708841655138No Hit
GTATCAACGCAGAGTACATGGGAACAC410.10313168154949062No Hit
TATCAACGCAGAGTACATGGGATGAG410.10313168154949062No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT410.10313168154949062No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC400.1006162746824299No Hit
TATCAACGCAGAGTACATGGGGAGGG400.1006162746824299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)