FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682445
Total Bases121.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT9990.14638542300112095No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA8280.1213284587036318No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT7790.11414839291078402No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT7450.1091663064422774No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG7420.10872671057740915No Hit
ATTTAAAGCCTAGTTAACGCATTTACTAAACGCAGACGAAAATGGAAAGA6890.10096051696473708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)