FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2036105
Total Bases349.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC216121.0614383835804146No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA214631.054120489856859No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC161870.7949982933100208No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA161720.7942615925995958No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC120450.5915706704713165No Hit
CCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTC110760.5439798045778582No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG104320.512350787410276No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG97750.4800832962936587No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT96470.4737967835646983No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA86770.4261568042905449No Hit
CCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTC86040.4225715274998097No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTAT80520.39546094135616777No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG71090.3491470233607795No Hit
TCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTT70740.3474280550364544No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC67950.33372542182254844No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT67470.3313679795491883No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT64450.31653573857929723No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA63620.31245932798161197No Hit
CTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTT62420.30656572229821155No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG59710.2932559961298656No Hit
CCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTT56620.27807996149510955No Hit
CTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGA56110.2755751790796644No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC55800.27405266427811925No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC52970.2601535775414333No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCC52180.25627362046652796No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT49730.24424084219625214No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA47590.23373057872752143No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG47550.2335341252047414No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG47520.2333867850626564No Hit
CAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTT47020.23093111602790622No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC46840.2300470751753962No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC46710.2294086012263611No Hit
GAGTTACAGCACCCCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCA45950.22567598429354085No Hit
TTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCA44680.21943858494527543No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA44650.2192912448031904No Hit
GTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCA42600.2092230017607147No Hit
AGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGC42180.20716023977152453No Hit
ATGTCAATGTGTCTGTTGTCATGGCGGAGGTGGACGGCACCTGCTACTGA42120.20686555948735452No Hit
GAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCC41940.20598151863484448No Hit
GACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCT41090.20180688127576918No Hit
GCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTCC39590.19443987417151867No Hit
GACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCT38740.19026523681244337No Hit
GGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGC38120.18722020720935315No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT37960.1864343931182331No Hit
ACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCC37390.1836349304186179No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT37290.18314379661166785No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA37160.18250532266263284No Hit
GGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAATAAAGTGAATCT35950.17656260359853743No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGG35080.1722897394780721No Hit
CCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATT34980.17179860567112207No Hit
CCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTC34600.16993229720471192No Hit
GGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCC34430.16909736973289688No Hit
GAAGACCATCGACCGCTTGGCGGGTAAACCCACCCATGTCAATGTGTCTG33840.16619968027189166No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG33390.16398957814061652No Hit
ACTTACTACTGTCAACAGAGTTACAGCACCCCGTACACTTTTGGCCAGGG32710.16064986825335628No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCT32250.1583906527413861No Hit
TGTTGGAGGAGAATGAATAAATAAAGTGAATCTTTGC32190.1580959724572161No Hit
GCCCCAAGGACGTGCTGGTTCGCTGGCTGCAGGGGTCACAGGAGCTGCCC31690.15564030342246593No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA31350.15397044847883581No Hit
CTACTGAGCCGCCCGCCTGTCCCCACCCCTGAATAAACTCCATGCTCCCC31280.1536266548139708No Hit
ACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGC30630.15043428506879558No Hit
GAATAAACTCCATGCTCCCCCAAGC30510.14984492450045553No Hit
GCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTAC30510.14984492450045553No Hit
GGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAG30300.14881354350586046No Hit
AGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCA30250.14856797660238544No Hit
GACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTG29600.1453756068572102No Hit
CCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAGAAGACC28860.14174121668577996No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC28830.14159387654369496No Hit
CCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTC28110.1380577131336547No Hit
GTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCA27930.13717367228114466No Hit
TCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGT27700.13604406452515955No Hit
GCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCC27620.13565115747959952No Hit
CTCCTCCTCCTTGGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAA27300.13407952929735942No Hit
GGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCT27050.13285169477998432No Hit
CACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGA26840.13182031378538925No Hit
CCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAG26730.13128006659774422No Hit
GCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAA25710.12627050176685387No Hit
CCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTG25710.12627050176685387No Hit
CACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCC25460.1250426672494788No Hit
GGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCC25410.12479710034600376No Hit
GAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGAC25390.12469887358461376No Hit
CCCCTGAATAAACTCCATGCTCCCCC25220.1238639461127987No Hit
GCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGAC25190.12371660597071368No Hit
AGCATACTGCGCGTGGCAGCCGAGGACTGGAAGAAGGGGGACACCTTCTC24870.1221449777884736No Hit
GGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGA24320.11944374185024839No Hit
CATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTG24100.11836324747495831No Hit
GTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGG23940.11757743338383826No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGT23790.11684073267341322No Hit
ATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAG23720.11649693900854818No Hit
GACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTAC23700.11639871224715817No Hit
CCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAG23290.11438506363866303No Hit
ACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACA23040.11315722912128795No Hit
CCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTTGGAGGAGAATGA22580.11089801360931778No Hit
GGAAGAAGGGGGACACCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCG22280.10942461218846769No Hit
CTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCT21950.10780387062553258No Hit
GGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGTGGCAGCCGAGG21850.10731273681858254No Hit
GAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCA21720.10667426286954748No Hit
CCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACA21670.10642869596607248No Hit
GAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGA21570.10593756215912245No Hit
CCCCTGAATAAACTCCATGCTCCCCCAAGC21450.1053482015907824No Hit
GCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAATAAAGTGAATCTT21310.10466061426105235No Hit
ACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCC21190.1040712536927123No Hit
AAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAG20710.10171381141935215No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAG20680.10156647127726713No Hit
CCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTT20660.10146824451587712No Hit
GACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTC20650.10141913113518213No Hit
ATGCTAATGTTGGAGGAGAATGAATAAATAAAGTGAATCTTTGC20630.10132090437379213No Hit
CCTTCTCCTGCATGGTGGGCCACGAGGCCCTGCCGCTGGCCTTCACACAG20610.10122267761240211No Hit
GCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCAT20580.10107533747031709No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT20570.1010262240896221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)