FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395638
Total Bases69.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT6770.1711160202002841No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG6570.166060894049611No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA6390.16151128051400523No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5920.14963173405992347No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG5650.1428073137565148No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5140.12991674207229842No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT4860.12283956546135608No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT4850.12258680915382242No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT4780.12081751500108684No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG4060.10261906085866372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)