FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3404362
Total Bases584.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG410831.2067753076787957No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT321060.9430841960990046No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC269280.7909852125009033No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA204460.6005824292481234No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC204220.5998774513403686No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG190220.558753740054671No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC149350.43870187717992387No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT146670.43082962387666174No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC126680.3721108389765836No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA124710.3663241453170961No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA124510.36573666372730046No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC115030.33789003637098525No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA112830.33142773888323274No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT106520.31289269472517905No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG105360.3094853015043641No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG104520.30701787882722226No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC95850.2815505519095795No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC94870.2786718921195807No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC89290.26228115576428124No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG88690.2605187109948942No Hit
CACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTG88610.2602837183589759No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT87310.256465088025304No Hit
ACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG85580.2513833722735714No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA85190.2502377831734698No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC82590.24260052250612596No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC81710.240015603511025No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC78690.2311446315051102No Hit
CCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCT78650.23102713518715107No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA75450.2216274297504202No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT72820.21390204684460704No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT72590.21322644301634197No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC71760.21078839441868988No Hit
CTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATA70790.20793910870818086No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG70690.207645367913283No Hit
GGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGG64040.18811160505257668No Hit
CCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAAT62580.1838229894470682No Hit
ATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTAT61010.1792112589671721No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG60510.1777425549926829No Hit
ATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTT58970.17321894675125618No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCA57120.16778474204564614No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA55640.16343737828115812No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA55230.16223304102207697No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA53900.1583262884499357No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA52730.15488952114963098No Hit
CCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTG52720.15486014707014117No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC52320.15368518389054983No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC50780.1491615756491231No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC50580.14857409405932742No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACA49990.14684102336943017No Hit
GGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATC49900.1465766566540221No Hit
TTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAG49640.14581293058728773No Hit
GTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTG49380.14504920452055334No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG49240.14463796740769636No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC48190.14155368906126903No Hit
CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT46840.13758818833014821No Hit
GGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCA46120.13547325460688375No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA44580.13094964636545703No Hit
GCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAAC43650.1282178569729071No Hit
GGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGA43020.12636728996505073No Hit
ACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCG42630.12522170086494913No Hit
GCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAA42460.12472234151362281No Hit
CCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCT42200.12395861544688842No Hit
GTACATGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAA42040.12348863017505188No Hit
GCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGG41670.12240178923392989No Hit
GTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGC40760.11972874800035954No Hit
CAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTC40650.11940563312597191No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC39070.11476452856658605No Hit
GTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGA38250.11235585404842377No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT36960.10856659779424163No Hit
CTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAG36230.10642228999148738No Hit
GTGTTCACTAGCAACCTCAAACAGACACCATGGTGCACCTGACTCCTGAG35690.10483608969903906No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT34870.10242741518087678No Hit
AGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCC34780.10216304846546873No Hit
CCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCC34580.10157556687567303No Hit
AGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGG34280.10069434449097951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)