FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491140
Total Bases86.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA9580.19505639939732053No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT9130.18589404243189314No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT8250.1679765443661685No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT8210.1671621126359083No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG7950.16186830638921693No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT7290.14843018283992343No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG7250.14761575110966324No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA5270.1073013804617828No Hit
GATTAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGTTTG5190.10567251700126237No Hit
GATTAAAAGACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAA5090.10363643767561184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)