FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2390188
Total Bases410.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG185130.7745415841766422No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT141270.591041374151322No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC116670.48812059971851585No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC94040.39344185478297106No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA86220.36072476307303025No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG85830.3590930922588516No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC65620.27453907391385113No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT64590.2702297894558922No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC61260.25629783096559766No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA57200.23931171941286625No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA55680.23295238700888798No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC55380.23169725561336596No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA51020.21345601266511255No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG46420.19421066460044148No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC45350.18973402928974625No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT44810.1874747927778066No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC44520.18626149909546863No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG43920.18375123630442458No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC43150.18052973238925138No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG42860.1793164387069134No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA42500.177810281032287No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA41710.17450510169074565No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC40470.1693172252559213No Hit
ACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG39610.16571918192209148No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC39330.1645477259529376No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT38970.16304156827831115No Hit
CACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTG38010.15902514781264068No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT36840.15413013537010478No Hit
CTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATA36750.1537535959514482No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC36530.15283316626139867No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA36210.1514943594395085No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA35220.14735242583428584No Hit
CCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCT35050.1466411847101567No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC35030.14655750928378855No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT34450.14413092191911264No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC33360.13957061118204928No Hit
ATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTT32980.13798077808105472No Hit
ATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTAT32300.1351358135845381No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG31050.12990609943652967No Hit
CCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAAT30300.1267682709477246No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG28820.12057628939648261No Hit
GGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGG27800.11630884265170774No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC27670.11576495238031487No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCA26630.11141383020917184No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA26220.10969848396862507No Hit
GTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCC24420.10216769559549292No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC24290.10162380532410002No Hit
CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT24010.10045234935494612No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC24010.10045234935494612No Hit
CCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCC23910.10003397222310545No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)