FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163300

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163300
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122504
Total Bases19.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGGAGTGGGCGGC10960.8946646640109711No Hit
GTATCAACGCAGAGTACATGGGGAGTGGGCGGC10450.8530333703389277No Hit
ACGCAGAGTACATGGGGAGTGGGCGGC8250.6734473976359956No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG7420.6056945079344348No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4070.33223404950042446No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA3950.3224384509893554No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG3870.3159080519819761No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG3680.3003983543394501No Hit
TATCAACGCAGAGTACATGGGGGGCGGC3500.2857049565728466No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC3430.27999085744138963No Hit
ATCAACGCAGAGTACATGGGGAGTGGGCGGC3380.2759093580617776No Hit
GTATCAACGCAGAGTACATGGGGGGCGGC3370.27509305818585517No Hit
GGTATCAACGCAGAGTACATGGGGAGTGGGCGGC3320.27101155880624306No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG3310.27019525893032065No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3190.2603996604192516No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT2990.24407366290080326No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC2800.2285639652582773No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2750.2244824658786652No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2620.21387056749167374No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2580.21060536798798404No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA2570.20978906811206163No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC2540.2073401684842944No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT2380.1942793704695357No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT2370.19346307059361328No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG2270.18530007183438907No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC2160.17632077319924247No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA2150.17550447332332006No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT2110.1722392738196304No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT2070.1689740743159407No Hit
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT2030.16570887481225102No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC2010.1640762750604062No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG1990.16244367530856135No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA1990.16244367530856135No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1970.1608110755567165No Hit
GTATCAACGCAGAGTACATGGGAGTGGGCGGC1910.15591327630118199No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1840.15019917716972506No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1800.1469339776660354No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA1780.14530137791419054No Hit
TATCAACGCAGAGTACATGGGATTGC1770.14448507803826813No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG1760.14366877816234572No Hit
TATCAACGCAGAGTACATGGGAGTGGGCGGC1760.14366877816234572No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA1740.14203617841050087No Hit
ATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTC1710.1395872787827336No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA1650.1346894795271991No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT1620.13224057989943186No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA1610.13142428002350942No Hit
ACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGT1600.130607980147587No Hit
GTATCAACGCAGAGTACATGGGATTGC1590.1297916802716646No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCA1590.1297916802716646No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC1570.12815908051981975No Hit
GACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGC1550.1265264807679749No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA1520.12407758114020767No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC1460.11917978188467317No Hit
ACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGG1450.11836348200875073No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1450.11836348200875073No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC1440.11754718213282832No Hit
TAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCA1430.11673088225690591No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1410.11509828250506106No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1400.11428198262913865No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG1390.11346568275321624No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT1390.11346568275321624No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC1380.1126493828772938No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACAT1380.1126493828772938No Hit
GACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATAC1360.11101678312544898No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC1350.11020048324952654No Hit
AGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAA1350.11020048324952654No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA1350.11020048324952654No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA1340.10938418337360413No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC1330.10856788349768172No Hit
TCAACGCAGAGTACATGGGGAGTGGGCGGC1320.10775158362175928No Hit
GTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGG1320.10775158362175928No Hit
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC1310.10693528374583687No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT1270.1036700842421472No Hit
GAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGA1270.1036700842421472No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC1260.10285378436622479No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA1260.10285378436622479No Hit
GTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACC1260.10285378436622479No Hit
TTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCA1240.10122118461437994No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT1230.10040488473845753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)