FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173859
Total Bases30.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA15190.8736965011877441No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC13440.7730402222490639No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA10910.627520001840572No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA10630.6114149972103832No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG9100.523412650481137No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC9040.5199615780603822No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG6230.35833635302170147No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC6110.351434208180192No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG6080.3497086719698146No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA5790.33302848860283335No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT5660.32555116502453135No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5490.3157731264990596No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT5420.31174687534151235No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC5420.31174687534151235No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC5380.30944616039434253No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT5160.29679222818490847No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA5070.29161561955377635No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC4860.2795368660811347No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT4460.25652971660943635No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4370.25135310797830424No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT4300.24732685682075706No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG4010.23064667345377576No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT3860.22201899240188888No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACGTTCTCCTGA3810.21914309871792662No Hit
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT3730.2145416688235869No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT3610.20763952398207744No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA3600.20706434524528494No Hit
TATCAACGCAGAGTACATGGGGGGCGGC3270.18808344693113385No Hit
GTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGG3250.18693308945754894No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC3180.18290683830000173No Hit
TTCTCGGCCTATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCA3120.17945576587924697No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT3110.17888058714245453No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT3100.17830540840566206No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG3090.17773022966886962No Hit
ATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTC3080.17715505093207715No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT3060.1760046934584922No Hit
ATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACAT3050.17542951472169976No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT3040.1748543359849073No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC3030.17427915724811485No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC3020.17370397851132238No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC2950.1696777273537752No Hit
ATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAA2950.1696777273537752No Hit
GTATCAACGCAGAGTACATGGGGGGCGGC2900.16680183366981288No Hit
ACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGT2890.16622665493302044No Hit
GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT2820.16220040377547323No Hit
ATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGA2800.16105004630188832No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCA2750.158174152617926No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC2750.158174152617926No Hit
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC2740.15759897388113356No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA2730.1570237951443411No Hit
CACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTC2720.15644861640754865No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC2710.15587343767075618No Hit
CTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCT2580.14839611409245423No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC2560.1472457566188693No Hit
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA2550.14667057788207685No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2550.14667057788207685No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA2520.14494504167169947No Hit
TCATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTA2520.14494504167169947No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT2500.14379468419811456No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG2470.1420691479877372No Hit
GTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTC2430.13976843304056735No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA2430.13976843304056735No Hit
CCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTA2400.13804289683018997No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2360.13574218188302015No Hit
AACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGA2360.13574218188302015No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC2330.13401664567264276No Hit
ATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAAT2320.13344146693585032No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC2290.13171593072547294No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC2280.13114075198868047No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2270.13056557325188803No Hit
GACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTC2270.13056557325188803No Hit
ATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTA2230.12826485830471818No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT2230.12826485830471818No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA2160.12423860714717098No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC2160.12423860714717098No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA2110.12136271346320868No Hit
GCTAAATCCCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCAT2100.12078753472641622No Hit
TAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCA2100.12078753472641622No Hit
CCCCTATTCTCAGGCTACACCCTAGACCAAACCTACGCCAAAATCCATTT2020.11618610483207657No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA2000.11503574735849166No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT1990.1144605686216992No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1960.11273503241132181No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG1960.11273503241132181No Hit
GTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACC1940.1115846749377369No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT1930.11100949620094444No Hit
GTCCTATCAATAGGAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTG1910.10985913872735953No Hit
ATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAA1870.10755842378018969No Hit
TCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATA1860.10698324504339723No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA1850.10640806630660478No Hit
ACGTTACTCGGACTACCCCGATGCATACACCACATGAAACATCCTATCAT1830.10525770883301987No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1820.10468253009622741No Hit
GTACTGAACCTACGAGTACACCGACTACGGCGGACTAATCTTCAACTCCT1810.10410735135943494No Hit
TCCTAGTCCTCATCGCCCTCCCATCCCTACGCATCCTTTACATAACAGAC1810.10410735135943494No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1780.10238181514905756No Hit
GACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATAC1780.10238181514905756No Hit
TATCAACGCAGAGTACATGGGGAGTGGGCGGC1780.10238181514905756No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1770.1018066364122651No Hit
ATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACAATTTT1760.10123145767547265No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA1750.10065627893868019No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGTTG1750.10065627893868019No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG1750.10065627893868019No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT1740.10008110020188773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)