FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281
Total Bases210.2 kbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAATCCTCAC90.702576112412178No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC90.702576112412178No Hit
GGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA60.468384074941452No Hit
ACCACCTCCACCACCATAGCCTCCTCTTCCACCAAAGTTTCCACCACGGC50.39032006245121No Hit
GAGACCATCTGAACTTGCAACCCATTGGTTTGACAAAAGCAATCCAAAAC50.39032006245121No Hit
CCCTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAAT50.39032006245121No Hit
CGTTAACCCTAACAAAAAAAACTCATACCCCCATTATGTAAAATCCATTG50.39032006245121No Hit
CTGTGGATGTGTCCCCCCTGCGCCGTGTGAACCAGGCCATCTGGCTGCTG40.312256049960968No Hit
GTCTGAGCAACACGTGGCGCACAGCAGTGTCAACGTAGTAGTTAACAGGG40.312256049960968No Hit
GCCCCATGTTCTGGTAGCCTCTTGGCACTGACCAGACTAGGAGCGCCTAT40.312256049960968No Hit
CTACTTTGACCGCGATGATGTGGCTTTGAAGAACTTTGCCAAATACTTTC40.312256049960968No Hit
TTCTGGAGCAACGTTTTCCCAGTATTCTTTCAGTTTTTGAACATCATGGT40.312256049960968No Hit
TAATAGATGAGGGTCATGCAGCAACTCAGCTCGTCAATCAACTCCATGAT40.312256049960968No Hit
CTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTACT40.312256049960968No Hit
GCTTGTTCCGGCTCCATCAAATCCAAGGCCAAGGCCTCCAGGTTCCTGAA40.312256049960968No Hit
GGATCTCAAAACTAAGGTTCTTATCCAGCTCTTTTATTTCACAGATGGGA40.312256049960968No Hit
GAGTTCTCATTAAAAGAAATTTGCTTTCATTGTTTTATTTCTTAATTGCT40.312256049960968No Hit
AGATACCTCTTTACGGACTCCACTTATGACTCCCTAAAGCCCATGTCGAA30.234192037470726No Hit
GATCAGTTGCTGAAGCTTATGAGTACTGTTGATCCCAAGTTGAATCATGT30.234192037470726No Hit
GAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAA30.234192037470726No Hit
CTTCATCTTGTGTTGAGACTTCGTGGTGGTGCTAAGAAAAGGAAGAAGAA30.234192037470726No Hit
GTGTGGCAACCACTGCAGTTTTAAAAACTGTTGCCTCCCAGCCAGAGCAA30.234192037470726No Hit
TGTCAGACCAGTTCTATCGCTACAAGATGCCCCGTCTGATTGCCAAGGTT30.234192037470726No Hit
GACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATAG30.234192037470726No Hit
GCCTTTCTATTAGCTCTTAGTAAGATTACACATGCAAGCATCCCCGTTCC30.234192037470726No Hit
CACTAAGCTTCTCTAGAACTCCACATCAGGCTCTCAACAAACTGGTATT30.234192037470726No Hit
CCATTCTCCTGCCTCAGCCTCTTGAGTAGCTGGGATTACTGGTGCCTGCC30.234192037470726No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT30.234192037470726No Hit
CCCCAGTCTCAGCCCTACTCCACTCAAGCACTATAGTTGTAGCAGGAATC30.234192037470726No Hit
CCCTAATTCCCCCCATCCTTACCACCCTCGTTAACCCTAACAAAAAAAAC30.234192037470726No Hit
AAATACCACCGGAACTGGTCGAATGAGGCACCTAAAAATTGTATACCGCA30.234192037470726No Hit
CCATACAATGGTACATATCAACCCTTAGTGAAGCCTTTTAAAAAACAAAC30.234192037470726No Hit
GATTGCACCAATGTACTTCAGCACAGGATCAGCCTTCAGCTGCTACGATC30.234192037470726No Hit
CTCTGCTTCTAGATCAATGCTGGGCTAGAAAAGTAAAGTCTGTTCTATCA30.234192037470726No Hit
GCACTTCAGCCTGGGCAACAGAGTAAGACTCAGTCTCAAAAACAAAAACA30.234192037470726No Hit
TCCCAATGCTATTAACTAACTTTACCTTGGCCTGGGCTAGGTCTCCATTG30.234192037470726No Hit
CCATTATGCAGAATCCACGCCAGTACAAGATCCCAGACTGGTTCTTGAAC30.234192037470726No Hit
AGAAAACGGAGACGCAGGAGAAGAACACCCTGCCGACCAAAGAGACCATT30.234192037470726No Hit
GCTGCAGCGGCTGCCCTGGACCTCAGTGGGAGGCGTGGCTGAGACCAACT30.234192037470726No Hit
ATCTAAATGCAACCCAATCAATCTTACTTGTTAATTTTCTAACCCACTCA30.234192037470726No Hit
ATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCACTT20.156128024980484No Hit
TGCTAGGCTAAGTTTTAAAAGAATTTTCTGTAGAGTTGGGGTCTCACCAT20.156128024980484No Hit
AGATAAGACCTCTTAAAAGGCAGCTGACTATATCATGTCACCATCATAGC20.156128024980484No Hit
GAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAA20.156128024980484No Hit
GGAGAAGAAGAATTGGCCATCTTAAAAAGCGGGTAAAAAACCTGGGTTAG20.156128024980484No Hit
GGAACCAACAGGACTACTGTAAATAAGTTCCTGTCTCTTGCCAACAAGAG20.156128024980484No Hit
GTAACTTACAGTTTCCTTTAAAAAAAAAAAAGTAAACCTGGCAGCTATAG20.156128024980484No Hit
ATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTT20.156128024980484No Hit
GGGTGACACAGCAAGACCCAGTCTCTAAAAAGAAATAATAAATAAATAAA20.156128024980484No Hit
ACCATCATGGCTTACATAAGTAAGATTAAATCTGATGGGCAACTGGAAAT20.156128024980484No Hit
GTCAAGAAATGGCAATAGCAATGTCCATCCATCCATACTTTGCCATAATG20.156128024980484No Hit
GTAATCCCAGTGCTTTGGGAGGCCGAGGCCGGAGGATCGCTTGAGGCCAG20.156128024980484No Hit
GTCTAGAGCTTGTCTCAATGGATCTAGAACTTCATCGCCCTCTGATCGCC20.156128024980484No Hit
GGCTAAGGCAGGAGAATCGCTTGAACCCGGGAGGCAGAGGCTGCAGTGAG20.156128024980484No Hit
GATCTATACTGTTGGAAAACGCTTCAAAGAGGCAAATAACTTCCTGTGGC20.156128024980484No Hit
ACAGAAGGTGGCAATAATGGCTTGCTCTTCATATTTTGGGTGGTTGGGGA20.156128024980484No Hit
AGATTAATGAGGGAAATCTTTATATTCTGTATAAAAACAAAAGCAAATTT20.156128024980484No Hit
AGACATTACAAGAAAACTACAGACCACCCTCTTTCCTGAATATAGATACA20.156128024980484No Hit
CAACACCCACTCCCTCTTAGCCAATATTGTGCCTATTGCCATACTAGTCT20.156128024980484No Hit
GTATTGGTATTGCGTCTCAAATTTTTCCCATTAACTTAAAAAAAAAAAAA20.156128024980484No Hit
GATAAACCCAAAGAAACCAAGTCTGAAGAGACCCTGGATGAGGGTCCACC20.156128024980484No Hit
TGAACTCCTGACCTCGTGACTCACCCTCCTGGCCTCCCAAAGTGCTGGGA20.156128024980484No Hit
GTCTTTAGCCATGCACAAACGGTAGTTTTGTGTGTTGGCTGCTCCACTGT20.156128024980484No Hit
CTCTCAGTATACTGTACTAAGTGCCGTACTTAGCATACCGTATAGTACAT20.156128024980484No Hit
GTTAAGACCATCAATGGATGAAGTTTTAAATACTCTTGAAAGTACTCAAG20.156128024980484No Hit
GTACACCTACATGTAGCATACAGATCATCTCAAAGACTAAACATATATAC20.156128024980484No Hit
TCCCTGGCCTCTGCCCACTAGACATCAGAAGCACTCCCCCAGTTGTGACA20.156128024980484No Hit
CGTCCTCCAAAGCCGCCTCTGCCTCCACCACGACCCCCGAAGCCACCTTC20.156128024980484No Hit
GTCCTGCGCCGTCAGCACAAGCCACGCTTCACCACCAAGAGGCCCAACAC20.156128024980484No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA20.156128024980484No Hit
GGTTCTTATAGTAACAAATAAAAAGCTGTTTTCTTC20.156128024980484No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCCTCACCGA20.156128024980484No Hit
AGACCAGCCTGACCAATATGGTGAAATCCTGTCTCAACTAAAAATACAAA20.156128024980484No Hit
TCTTGGCACTGACCAGACTAGGAGCGCCTATGTTTGGCACAGATGCTGAG20.156128024980484No Hit
AGCCTGACCAACATGGAGAAACCCCATCTCTACTAAAAATAAAAAATTAG20.156128024980484No Hit
GGCCACCAGGGCTTTGGAGATGGACTGACGGATAGCATAAATCTGGGCCA20.156128024980484No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC20.156128024980484No Hit
GCAGAGGGGCGAATGAAGAGAGATTGGTTAATGGGTGCAAAAATAGAGTT20.156128024980484No Hit
GCATGATATTTGGTGCCTTGTCAAAGCTTGATAAACAGGGTCCAGTCTAA20.156128024980484No Hit
GGGTCTCCGCTGTGGATCATCAGGCCATCCACAAACTTCATGGATTTAGC20.156128024980484No Hit
ATCTATTACAGTTCCCTCAACACCTATCCAACCTATAGCAGAAGAAAGAA20.156128024980484No Hit
CACACACCCACACACACACAAAGTGACTAATTCCTTCCCCTACCCCCAGT20.156128024980484No Hit
GATCCAGACTATGACCCAAAGAAGGATCAAAACCCAGCAGAGTGCAAGCA20.156128024980484No Hit
AACTAATATTTCACTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCC20.156128024980484No Hit
GCTACTCAGGAGGCTGAAAACAGGAGAATCGCTTAAACCTGTGAGGCAGA20.156128024980484No Hit
CTTCGGGAGGCCAAGGTGGGTGGATCACTTGAGGGCAGGAGATCGAGACC20.156128024980484No Hit
GCTTCCACCCCCTAGCAGAAAATAGCCCACTAATCCAAACTCTAACACTA20.156128024980484No Hit
GCCATTAAGAAGAAGGACGAGCTGGAGCGTGTGGCCAAGTCCAACCGCTG20.156128024980484No Hit
ATTATCTTCTAATCAATCAAATATTTTAGCTGAACTTTAGGGAAAAAAAA20.156128024980484No Hit
CACTCATTCTCAACCCCCTGACAAAACACATAGCCTACCCCTTCCTTGTA20.156128024980484No Hit
GCTGTATTGCCAATGTATTTGCTCTAATTTTAGACAGTTGGAGGAAAATT20.156128024980484No Hit
GCAGCGTTCAACTCCCGGACTCAAGTTATTCTTCTCCTCCCCAGTAGCTA20.156128024980484No Hit
CTATTGGGTTCATTATTGGAATTAACCTGGTGCTGGATATTTTCAAATTG20.156128024980484No Hit
AATGTGGGAGGGAAAGGCAACTCTGGTTTGAGGTGTATTCCATTCCTGTG20.156128024980484No Hit
GGACACGTGGAGTAACAAGACGCTGAATCTTGGGTGCTTTGGTCCTAGGT20.156128024980484No Hit
CCTCTGAGACCCACCTTGCTCATAAACAAAATGCCCATGTTGGTCCTCTG20.156128024980484No Hit
CTACAAGGGCAAGAAGTACAAGCCCCTGGACCTGCGGCCTAAGAAGACAC20.156128024980484No Hit
AGTACACATAGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.156128024980484No Hit
ATGTTGTCTTGGTGATCCAGTTTGGTCCCCTTGGGAGTTTCTGTGCCAAA20.156128024980484No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG20.156128024980484No Hit
GTTTCATCCTCGCCTTAGCATGATTTATCCTACACTCCAACTCATGAGAC20.156128024980484No Hit
GCCCCTCCCCTACTCTCACTGTAATTTATGGACCCTGCCCGCCTGCGTGT20.156128024980484No Hit
GTGGTTACCATGTCTCAAGAATTTTCAGGTTACAGATACTCTCCAATGAA20.156128024980484No Hit
GCAATAGCAAGTGACAAAAATGGCTAACTGGAGAGGAAAAAAAATAGGAT20.156128024980484No Hit
CAGATAAGCCCATTTTGGCCGGGTGCGGTGGCTCACACCTGTAATCCCAG20.156128024980484No Hit
GAGCTGCACAATTCAGTGAACCAATGTTTTCCAAATGACACATGTGTAGG20.156128024980484No Hit
TTCCATGGCCCTGCATCAGGGAACTTGGGTTTATCCTTCATGTTCTCACC20.156128024980484No Hit
CATCAAGGGAATGGGTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACC20.156128024980484No Hit
CTCATCATTCTTTTAAAAATTTAGGAAAGTATTTTCTTAAAATGCATTCA20.156128024980484No Hit
GCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGG20.156128024980484No Hit
GCTCTAAGATAGGTGTTTGTCCTTTTACCATCGAGCTACTTCCCATAATA20.156128024980484No Hit
ACTGAAGAGGCTGACATGGGAGGACCACTTGAGCCCGGGAGATCAAAGCT20.156128024980484No Hit
TTGTACTGTAGCGTGCGCGGCTCAATCATCTCCAGGGGCCGCCCGTTCAC20.156128024980484No Hit
CCCTATGATTTCATTTCTAACCCAACCAATCAACACTCCCCATTTCCTAG20.156128024980484No Hit
GATGGCTCACGCCTGTGGTCTCAGCGCTTTGGGAGGCCGAGGTGGGCAGA20.156128024980484No Hit
CTTTACATCCAAACATCACTTTGGCTTCGAAGCCGCCGCCTGATACTGGC20.156128024980484No Hit
TCTTAAACCCTGCAACATGAGGAACTCTAAATACATTAAATATTGTTACA20.156128024980484No Hit
GTCTCGCTCTGTCGCCCAGGCTGGGGTACAATGGCACAATCACAGCTCAC20.156128024980484No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT20.156128024980484No Hit
CTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCACA20.156128024980484No Hit
ACTCTCCCTTCTTTAGCTACCAGCTCCACTTGGAAGTCTTCAAATTTCTT20.156128024980484No Hit
CTGTTAAGAGTGAAAAGTTAGACAAGCAAGCTAATATGTACTCATAGGCA20.156128024980484No Hit
GGCTGGAGTGAAGCGGTGCGATCTCGGCTCACTGCAAGCTCCGCCTCCCG20.156128024980484No Hit
GCCTAGCCACACCCCCACGGGAAACAGCAGTGATTAACCTTTAGCAATAA20.156128024980484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)