FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26851
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4091.5232207366578525No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3701.3779747495437786No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT2480.9236155078023165No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2000.7448512159696101No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1720.6405720457338646No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1120.4171166809429816No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1070.39849540054374144No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1060.3947711444638934No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1050.3910468883840452No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1040.3873226323041973No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1000.37242560798480506No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG940.35008007150571674No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA930.3463558154258687No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT920.34263155934602063No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA890.33145879110647647No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC840.31283751070723625No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT820.3053889985475401No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT820.3053889985475401No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA800.29794048638784404No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT790.294216230307996No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA770.2867677181482999No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT770.2867677181482999No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT740.27559494990875577No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT740.27559494990875577No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC730.27187069382890766No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG700.26069792558936355No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG700.26069792558936355No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG660.24580090126997134No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT630.23462813303042718No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT620.23090387695057912No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG620.23090387695057912No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG600.22345536479088302No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA590.21973110871103496No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC590.21973110871103496No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT550.20483408439164277No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA550.20483408439164277No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC540.20110982831179472No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG540.20110982831179472No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC540.20110982831179472No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC540.20110982831179472No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC530.1973855722319467No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA520.19366131615209864No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC500.18621280399240253No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG470.17504003575285837No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG470.17504003575285837No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT430.16014301143346615No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA430.16014301143346615No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT430.16014301143346615No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT430.16014301143346615No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG420.15641875535361813No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA420.15641875535361813No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT420.15641875535361813No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG420.15641875535361813No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA420.15641875535361813No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG410.15269449927377005No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG410.15269449927377005No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC410.15269449927377005No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC400.14897024319392202No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT390.14524598711407397No Hit
CGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCC390.14524598711407397No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA380.1415217310342259No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG380.1415217310342259No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT380.1415217310342259No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT380.1415217310342259No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA370.13779747495437789No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC370.13779747495437789No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA370.13779747495437789No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC370.13779747495437789No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG360.1340732188745298No Hit
CTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGCAG360.1340732188745298No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC350.13034896279468178No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA340.12662470671483372No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG320.1191761945551376No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA320.1191761945551376No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC310.11545193847528956No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA310.11545193847528956No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC310.11545193847528956No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC300.11172768239544151No Hit
TCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCG300.11172768239544151No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT300.11172768239544151No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC300.11172768239544151No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA290.10800342631559347No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT290.10800342631559347No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC290.10800342631559347No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG290.10800342631559347No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC290.10800342631559347No Hit
GAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCC280.1042791702357454No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA280.1042791702357454No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT280.1042791702357454No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT280.1042791702357454No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG280.1042791702357454No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA280.1042791702357454No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT280.1042791702357454No Hit
TGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGC280.1042791702357454No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG280.1042791702357454No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC270.10055491415589736No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT270.10055491415589736No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT270.10055491415589736No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC270.10055491415589736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)