FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163446

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163446
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724977
Total Bases126.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG114071.573429226030619No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC101181.3956304820704657No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT96751.3345250952788847No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG63620.8775450807404925No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC59230.8169914355903705No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC55550.7662312045761452No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA53130.7328508352678774No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT48890.6743662212732265No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC47750.658641584491646No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC47310.6525724264355973No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA46510.6415375936064178No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC41840.5771217569660831No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT41490.5722940176033171No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA40210.55463828507663No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC38500.5310513299042591No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC38030.5245683656171161No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA37370.5154646285330431No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC36730.5066367622696996No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT32220.4444278921952006No Hit
GTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTT31130.42939293246544374No Hit
CTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAA29950.41311655404240416No Hit
GGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAA29330.40456455859979007No Hit
GTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCG29300.4041507523686958No Hit
TCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAG29270.40373694613760164No Hit
CACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTG28720.3961504985675408No Hit
CCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGC28690.3957366923364465No Hit
CCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTG28560.3939435320017049No Hit
GTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCT28480.3928400487187869No Hit
CTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTG28040.3867708906627383No Hit
ACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTG27930.3852536011487261No Hit
CTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCA27520.3795982493237717No Hit
CCTTTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCT26640.3674599332116743No Hit
ATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTATCAC26370.36373567713182625No Hit
CACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTA25850.35656303579285964No Hit
ATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTT25170.3471834278880571No Hit
CCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATG24940.34401091344966805No Hit
GCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAA24240.33435543472413604No Hit
GGTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGC24090.33228640356866496No Hit
CTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATA23900.32966563077173483No Hit
ATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTAT22430.30938912544811764No Hit
CCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAAT21660.29876809885003247No Hit
GCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGA20590.28400900994100503No Hit
GGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATC19960.2753190790880262No Hit
CCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCAT19690.27159482300817817No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA19110.2635945692070231No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC18830.25973237771681035No Hit
GGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGG18620.2568357340991507No Hit
CAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTC18270.25200799473638474No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA18060.24911135111872515No Hit
GCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC17680.24386980552486495No Hit
GCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAATTTCT17650.2434559992937707No Hit
CTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCA17620.24304219306267646No Hit
GTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAAC17610.24290425765231172No Hit
GTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCC17360.23945587239319316No Hit
CCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCT16570.2285589749743785No Hit
TTACTGCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCC16440.22676581463963685No Hit
CACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTC15670.21614478804155166No Hit
CCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCC15400.2124205319617036No Hit
CCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAAT14690.2026271178258069No Hit
GGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCA14560.20083395749106525No Hit
GTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTG14320.1975235076423114No Hit
GCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCA14050.19379925156246336No Hit
TGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACA14050.19379925156246336No Hit
CCTTTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTG13890.19159228499662748No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC13360.18428170824729612No Hit
TCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC13180.18179887086073077No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC12610.17393655246994041No Hit
AGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGG12560.17324687541811673No Hit
GCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCA11800.16276378423039628No Hit
CCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCGTTACTGCCCTGTGG11780.16248791340966678No Hit
GGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGA11740.16193617176820782No Hit
CACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTT11730.16179823635784307No Hit
CCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCC11650.16069475307492512No Hit
CCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCA11570.1595912697920072No Hit
GCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACCTGGACAAC11540.15917746356091295No Hit
GCCCTGTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGG11060.15255656386340533No Hit
GGCTCACCTGGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGC10900.15034959729756944No Hit
CCTCCCCTCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTG10720.1478667599110041No Hit
TTGGGGATCTGTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAG10160.1401423769305785No Hit
ACCTCAAACAGACACCATGGTGCACCTGACTCCTGAGGAGAAGTCTGCCG9770.13476289592635352No Hit
GTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGAT9680.13352147723307084No Hit
TCACTAAGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGT9660.13324560641234134No Hit
CGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGC9620.13269386477088238No Hit
CCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGCC9590.13228005853978814No Hit
GGACAACCTCAAGGGCACCTTTGCCACACTGAGTGAGCTGCACTGTGACA9580.13214212312942342No Hit
TTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC9530.1314524460775997No Hit
CCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGC9380.12938341492212857No Hit
GATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGC9360.12910754410139907No Hit
GTGTGGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCT8880.12248664440389144No Hit
GCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTGCTGGTCTGTG8830.12179696735206771No Hit
GGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGC8720.12027967783805554No Hit
AGTTGGTGGTGAGGCCCTGGGCAGGCTGCTGGTGGTCTACCCTTGGACCC8540.11779684045149018No Hit
GCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAA8400.1158657447063838No Hit
GGCTGCTGGTGGTCTACCCTTGGACCCAGAGGTTCTTTGAGTCCTTTGGG8350.11517606765456007No Hit
GTGGGGCAAGGTGAACGTGGATGAAGTTGGTGGTGAGGCCCTGGGCAGGC8290.1143484551923716No Hit
GGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAA8280.11421051978200687No Hit
CTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAG8210.11324497190945368No Hit
CATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATTGC8200.11310703649908893No Hit
TCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTG8060.11117594075398254No Hit
TCTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGT7900.10896897418814666No Hit
ATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTC7870.10855516795705243No Hit
ACCCAGAGGTTCTTTGAGTCCTTTGGGGATCTGTCCACTCCTGATGCTGT7860.1084172325466877No Hit
GTTCACCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGA7700.1062102659808518No Hit
GCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTTGC7680.10593439516012233No Hit
GTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGG7600.10483091187720438No Hit
CTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGG7570.10441710564611016No Hit
CTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACG7410.10221013908027426No Hit
GGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCA7400.10207220366990953No Hit
CCCGTACCCCCGTGGTCTTTGAATAAAGTCTGAGTGGGCGGC7340.10124459120772107No Hit
GGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGATGGCCTGGCTCACC7320.10096872038699159No Hit
GCCTAATAAAAAACATTTATTTTCATTGC7310.10083078497662684No Hit
CTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCCAA7250.1000031725144384No Hit
AAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATA7250.1000031725144384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)