FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296586
Total Bases51.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC7400.24950604546404753No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG7150.24107678717134323No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT5950.20061634736636252No Hit
TCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGACCTCCAAATA5790.1952216220590318No Hit
CTGTGAGCACCGTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTA5660.19083840774682556No Hit
GTGCTGACCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCC5230.17634008348337413No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC4970.16757365485896167No Hit
CCCCTGCGGTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACC4900.16521346253700445No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4360.14700626462476316No Hit
GTGCACGCCTCCCTGGACAAGTTCCTGGCTTCTGTGAGCACCGTGCTGAC4100.13823983600035067No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4070.13722832500522614No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG4000.13486813268326894No Hit
CCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGCTGGGCCTCCC3810.12846189638081365No Hit
CCTCCCCTCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTG3670.12374151173689925No Hit
TCCTTGCACCGGCCCTTCCTGGTCTTTGAATAAAGTCTGAGTGGGCGGC3620.1220556600783584No Hit
CCCGTACCCCCGTGGTCTTTGAATAAAGTCTGAGTGGGCGGC3400.11463791278077859No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC3390.11430074244907042No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT3360.11328923145394591No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC3260.10991752813686419No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA3220.10856884681003148No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC3090.10418563249782525No Hit
CCTCCAAATACCGTTAAGCTGGAGCCTCGGTAGCCGTTCCTCCTGCCCGC3060.10317412150270074No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC3040.10249978083928439No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC3000.1011510995124517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)