FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36720
Total Bases5.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2540.6917211328976035No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2220.6045751633986929No Hit
GTATCAACGCAGAGTACATGGGGGGG1420.3867102396514161No Hit
TATCAACGCAGAGTACATGGGGGGG1350.3676470588235294No Hit
GGTATCAACGCAGAGTACATGGGGG1260.3431372549019608No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1120.30501089324618735No Hit
GTATCAACGCAGAGTACATGGGGGG890.24237472766884532No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA790.21514161220043573No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG790.21514161220043573No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA740.20152505446623092No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA700.1906318082788671No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC670.18246187363834424No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA610.1661220043572985No Hit
GTATCAACGCAGAGTACATGGGGAG600.16339869281045752No Hit
GTATCAACGCAGAGTACATGGGGTG570.15522875816993464No Hit
GGTATCAACGCAGAGTACATGGGGGGG530.1443355119825708No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT530.1443355119825708No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC520.14161220043572986No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT510.1388888888888889No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT500.13616557734204793No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT470.12799564270152505No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA450.12254901960784313No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG450.12254901960784313No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG430.11710239651416122No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA400.10893246187363835No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA390.10620915032679738No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC380.10348583877995642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)