FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25087
Total Bases3.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1660.6616972934189023No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1040.41455734045521586No Hit
TATCAACGCAGAGTACATGGGGGGG910.3627376728983138No Hit
GTATCAACGCAGAGTACATGGGGGG860.34280703153027464No Hit
GGTATCAACGCAGAGTACATGGGGG730.29098736397337266No Hit
GTATCAACGCAGAGTACATGGGGGGG580.231195439869255No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA550.21923705504843147No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA490.1953202854067844No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC470.1873480288595687No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG400.15944513094431378No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA370.14748674612349028No Hit
GTATCAACGCAGAGTACATGGGGAG360.1435006178498824No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA350.13951448957627458No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG340.13552836130266674No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT340.13552836130266674No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA310.12356997648184319No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG300.11958384820823535No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA300.11958384820823535No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG290.1155977199346275No Hit
GTATCAACGCAGAGTACATGGGGTG280.11161159166101967No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA270.10762546338741182No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT260.10363933511380397No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT260.10363933511380397No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC260.10363933511380397No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT260.10363933511380397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)