FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008163839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008163839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24885
Total Bases3.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2751.105083383564396No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2551.024713682941531No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1090.4380148683946153No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1010.4058669881454691No Hit
GGTATCAACGCAGAGTACATGGGGG770.30942334739803096No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA770.30942334739803096No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG720.28933092224231466No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC680.27325698211774163No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA640.25718304199316855No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT580.233072131806309No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT570.22905364677516574No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT530.2129797066505927No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC500.20092425155716293No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG460.1848503114325899No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA450.18083182640144665No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA440.17681334137030338No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT440.17681334137030338No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC380.15270243118344384No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT380.15270243118344384No Hit
GTATCAACGCAGAGTACATGGGGGG370.14868394615230057No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT350.14064697609001406No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG340.13662849105887082No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG330.13261000602772754No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT320.12859152099658427No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC320.12859152099658427No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG320.12859152099658427No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC310.12457303596544102No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA310.12457303596544102No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG300.12055455093429776No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG300.12055455093429776No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT300.12055455093429776No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT290.1165360659031545No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG290.1165360659031545No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA280.11251758087201125No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT280.11251758087201125No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC280.11251758087201125No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC270.108499095840868No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT260.10448061080972475No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC250.10046212577858146No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)