FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37830
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3720.9833465503568596No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3710.9807031456515992No Hit
GTATCAACGCAGAGTACATGGGGGG1840.48638646576790906No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1840.48638646576790906No Hit
GGTATCAACGCAGAGTACATGGGGG1770.46788263283108644No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1580.4176579434311393No Hit
GTATCAACGCAGAGTACATGGGGGGG1450.3832936822627544No Hit
TATCAACGCAGAGTACATGGGGGGG1220.32249537404176576No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1130.2987047316944224No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1030.27227068464181864No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG920.24319323288395453No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC910.24054982817869416No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT900.23790642347343377No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA860.2273328046523923No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT830.21940259053661115No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA780.2061855670103093No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC770.20354216230504893No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA740.19561194818926778No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA730.1929685434840074No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG720.19032513877874702No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC700.18503832936822626No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA670.17710811525244516No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA660.1744647105471848No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG660.1744647105471848No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC640.16917790113666403No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT620.16389109172614327No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC620.16389109172614327No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG610.16124768702088288No Hit
TATCAACGCAGAGTACATGGGACTG610.16124768702088288No Hit
GTATCAACGCAGAGTACATGGGGTG600.1586042823156225No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG580.15331747290510178No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT530.1401004493787999No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC530.1401004493787999No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG530.1401004493787999No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT520.13745704467353953No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA510.13481363996827914No Hit
GTATCAACGCAGAGTACATGGGACTG510.13481363996827914No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT510.13481363996827914No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT510.13481363996827914No Hit
GTATCAACGCAGAGTACATGGGGAG490.12952683055775838No Hit
GTATCAACGCAGAGTACATGGGAGG450.11895321173671689No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA440.11630980703145652No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT440.11630980703145652No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA440.11630980703145652No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT440.11630980703145652No Hit
GAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTT430.11366640232619615No Hit
GGTATCAACGCAGAGTACATGGGGGG430.11366640232619615No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC420.11102299762093576No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC420.11102299762093576No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG410.1083795929156754No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG410.1083795929156754No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC410.1083795929156754No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT400.10573618821041501No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT390.10309278350515465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)