FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43109
Total Bases5.7 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4260.9881927207775639No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2740.6355981349602171No Hit
TATCAACGCAGAGTACATGGGACTG2640.6124011227353916No Hit
GTATCAACGCAGAGTACATGGGACTG2420.5613676958407757No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1670.38739010415458486No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1410.32707787237003877No Hit
GGTATCAACGCAGAGTACATGGGGG1080.2505277320281148No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA910.2110928112459115No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG870.20181400635598135No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG860.19949430513349883No Hit
TATCAACGCAGAGTACATGGGGGGTTTTACTG850.19717460391101624No Hit
GTATCAACGCAGAGTACATGGGGGG840.19485490268853373No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT810.18789579902108608No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC810.18789579902108608No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA780.18093669535363846No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA750.1739775916861908No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC700.1623790855737781No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT680.157739683128813No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG660.1531002806838479No Hit
GTATCAACGCAGAGTACATGGGGGGTTTTACTG660.1531002806838479No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG640.14846087823888282No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT590.13686237212647012No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG580.13454267090398758No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT570.13222296968150501No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG530.12294416479157483No Hit
ACGCAGAGTACATGGGGGGTTTTACTG520.1206244635690923No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC500.11598506112412721No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT500.11598506112412721No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT500.11598506112412721No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC490.11366535990164467No Hit
GTATCAACGCAGAGTACATGGGGTG490.11366535990164467No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT490.11366535990164467No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC480.11134565867916213No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT480.11134565867916213No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC480.11134565867916213No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC470.10902595745667958No Hit
GTATCAACGCAGAGTACATGGGATG470.10902595745667958No Hit
GGTATCAACGCAGAGTACATGGGACTG470.10902595745667958No Hit
TATCAACGCAGAGTACATGGGATTG460.10670625623419705No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA460.10670625623419705No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA450.1043865550117145No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG450.1043865550117145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)