FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164430

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164430
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286216
Total Bases49.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC7050.24631746652877548No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG6190.21627022947703833No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA4860.16980182799004948No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT4220.14744109343991949No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4050.1415015233250412No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG3930.13730888559689186No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA3700.1292729966179389No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC3690.12892361014059311No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA3580.12508035888978952No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA3490.1219358805936775No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3470.12123710763898593No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT3200.11180367275064985No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC3140.10970735388657518No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG3130.10935796740922939No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC3070.1072616485451547No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC3000.10481594320373425No Hit

[OK]Adapter Content

Adapter graph