FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111422
Total Bases14.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC6090.5465706951948448No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC5970.5358008292796754No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA5400.484643966182621No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5350.48015652205130044No Hit
TATCAACGCAGAGTACATGGGGGGG4680.4200247706916049No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA4210.3778427958571916No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA4130.3706628852470787No Hit
GTATCAACGCAGAGTACATGGGGGGG3990.3580980416793811No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA3770.3383532875015706No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA3570.32040351097628833No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC3410.30604368975606255No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG3280.29437633501462906No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG3220.2889914020570444No Hit
GGTATCAACGCAGAGTACATGGGGG3210.28809391323078026No Hit
GTATCAACGCAGAGTACATGGGGGG3210.28809391323078026No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2590.23244960600240527No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2500.22437220656602824No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT2180.19565256412557666No Hit
GTATCAACGCAGAGTACATGGGGAG2140.19206260882052018No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA2130.19116511999425606No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA2040.18308772055787906No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA2020.18129274290535083No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA2020.18129274290535083No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA2000.1794977652528226No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1870.16783041051138914No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG1860.16693292168512502No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1790.16065049990127622No Hit
GTATCAACGCAGAGTACATGGGGTG1770.158855522248748No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1760.15795803342248388No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC1720.15436807811742742No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA1700.15257310046489922No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1680.150778122812371No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1660.14898314515984276No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1620.14539318985478633No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCG1450.13013587980829638No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1450.13013587980829638No Hit
CCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTCCCGCGG1420.12744341332950404No Hit
TATCAACGCAGAGTACATGGGGGGGACCTCC1400.1256484356769758No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG1400.1256484356769758No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA1370.12295596919818348No Hit
GTATCAACGCAGAGTACATGGGGGGGACCTCC1360.12205848037191937No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA1360.12205848037191937No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT1310.1175710362405988No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1290.11577605858807058No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1280.11487856976180646No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1270.11398108093554236No Hit
CCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAG1250.11218610328301412No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1240.11128861445675No Hit
CCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCC1230.1103911256304859No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA1230.1103911256304859No Hit
GGTATCAACGCAGAGTACATGGGGGGG1220.10949363680422179No Hit
CCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGT1200.10769865915169355No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1190.10680117032542945No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTG1190.10680117032542945No Hit
GTATCAACGCAGAGTACATGGAGAG1170.10500619267290122No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC1170.10500619267290122No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT1160.10410870384663712No Hit
TAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCG1140.10231372619410888No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT1130.10141623736784476No Hit
GTATCAACGCAGAGTACATGGGATG1130.10141623736784476No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1120.10051874854158066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)