FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18067
Total Bases3.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3932.175236619250567No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3742.0700725078873083No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1440.7970332650689101No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1310.7250788730835224No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1270.7029390601649416No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1140.6309846681795539No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1080.5977749488016827No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1000.553495322964521No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT950.5258205568162949No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA910.5036807438977141No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA880.48707588420877845No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG820.45386616483090714No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA800.4427962583716168No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT780.43172635191232633No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT750.4151214922233907No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT750.4151214922233907No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT740.40958653899374553No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG720.3985166325344551No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA690.38191177284551947No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG670.37084186638622907No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC650.3597719599269386No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT620.343167100238003No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT600.33209719377871255No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG570.31549233408977695No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC550.3044224276304865No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG550.3044224276304865No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT540.29888747440084135No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA540.29888747440084135No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC540.29888747440084135No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT540.29888747440084135No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG530.2933525211711961No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG520.2878175679415509No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT510.2822826147119057No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG510.2822826147119057No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC500.2767476614822605No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC500.2767476614822605No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT480.2656777550229701No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA480.2656777550229701No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG470.26014280179332483No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG430.238002988874744No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG420.2324680356450988No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG400.2213981291858084No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT390.21586317595616317No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT390.21586317595616317No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT380.21032822272651794No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA380.21032822272651794No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG380.21032822272651794No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG370.20479326949687277No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA370.20479326949687277No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT360.19925831626722754No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC360.19925831626722754No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA350.19372336303758234No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC350.19372336303758234No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT350.19372336303758234No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT350.19372336303758234No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT340.1881884098079371No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT340.1881884098079371No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC340.1881884098079371No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC340.1881884098079371No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT340.1881884098079371No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC340.1881884098079371No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT340.1881884098079371No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG330.1826534565782919No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA330.1826534565782919No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG330.1826534565782919No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA320.1771185033486467No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG310.1715835501190015No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT310.1715835501190015No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC310.1715835501190015No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT300.16604859688935628No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT300.16604859688935628No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT300.16604859688935628No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC300.16604859688935628No Hit
GCTTACAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGG290.16051364365971107No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG290.16051364365971107No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA290.16051364365971107No Hit
GATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTT290.16051364365971107No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT290.16051364365971107No Hit
CCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTAT280.15497869043006587No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG280.15497869043006587No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA280.15497869043006587No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA280.15497869043006587No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA280.15497869043006587No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA270.14944373720042067No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT270.14944373720042067No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC260.14390878397077544No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC260.14390878397077544No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC260.14390878397077544No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC260.14390878397077544No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT250.13837383074113024No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA250.13837383074113024No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC250.13837383074113024No Hit
TCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCT250.13837383074113024No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG250.13837383074113024No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC250.13837383074113024No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT240.13283887751148504No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA240.13283887751148504No Hit
GTACTGGTAGGCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAAC240.13283887751148504No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA240.13283887751148504No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA230.12730392428183981No Hit
CCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCT230.12730392428183981No Hit
GGTCAGAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATA230.12730392428183981No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT230.12730392428183981No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT230.12730392428183981No Hit
AACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCC220.12176897105219461No Hit
GCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAAC220.12176897105219461No Hit
ACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGA220.12176897105219461No Hit
TTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGC220.12176897105219461No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG220.12176897105219461No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC220.12176897105219461No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA220.12176897105219461No Hit
GTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCC220.12176897105219461No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA220.12176897105219461No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT210.1162340178225494No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC210.1162340178225494No Hit
GTGTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGT210.1162340178225494No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG210.1162340178225494No Hit
GAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGAGGAAAACTGCGACTGT210.1162340178225494No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT210.1162340178225494No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG210.1162340178225494No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC210.1162340178225494No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG200.1106990645929042No Hit
CAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTT200.1106990645929042No Hit
CCATAAGACATATGGGCAATCATTATTGGGGTATCTAACTTTAAGTTTGG200.1106990645929042No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG200.1106990645929042No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT200.1106990645929042No Hit
CCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATT200.1106990645929042No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC200.1106990645929042No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG200.1106990645929042No Hit
GTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAGACCTCCTGTTTG190.10516411136325897No Hit
GGGTATGCTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGG190.10516411136325897No Hit
GGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTT190.10516411136325897No Hit
AGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCT190.10516411136325897No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT190.10516411136325897No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT190.10516411136325897No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)