FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35571
Total Bases5.8 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4931.3859604734193585No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4291.2060386269714092No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2310.6494054145230665No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1710.4807286834781142No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC1710.4807286834781142No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1620.4554271738213714No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1410.39639031795563806No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1370.38514520255264123No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1310.36827752944814596No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1220.34297601979140313No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1060.2979955581794158No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT950.26707149082117454No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG920.25863765426892693No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT870.24458126001518093No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA850.2389587023136825No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA830.23333614461218408No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT830.23333614461218408No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG820.23052486576143486No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT810.2277135869106857No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT750.21084591380619044No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC740.20803463495544122No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG700.19678951955244442No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT680.191166961850946No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC670.18835568300019678No Hit
GGTATCAACGCAGAGTACATGGGGG650.18273312529869837No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC640.17992184644794917No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG630.17711056759719998No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA630.17711056759719998No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC620.17429928874645076No Hit
GTATCAACGCAGAGTACATGGGGGG610.17148800989570157No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT610.17148800989570157No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA600.16867673104495234No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG590.16586545219420312No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC570.16024289449270473No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA570.16024289449270473No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT570.16024289449270473No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG560.15743161564195554No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG540.15180905794045713No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT530.1489977790897079No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA520.14618650023895868No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT510.1433752213882095No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC500.1405639425374603No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG500.1405639425374603No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT490.1377526636867111No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG490.1377526636867111No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT490.1377526636867111No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG480.13494138483596188No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA480.13494138483596188No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC470.13213010598521266No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT470.13213010598521266No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC470.13213010598521266No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT470.13213010598521266No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA460.12931882713446347No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT450.12650754828371427No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG450.12650754828371427No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAACCACAGTTT450.12650754828371427No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG440.12369626943296505No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC440.12369626943296505No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT440.12369626943296505No Hit
CGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACC440.12369626943296505No Hit
GCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAG440.12369626943296505No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG420.11807371173146665No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC400.11245115402996823No Hit
CCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAA400.11245115402996823No Hit
CTCTAGAGGAGCCTGTTCTGTAATCGATAAACCCCGATCAACCTCACCAC400.11245115402996823No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG390.10963987517921903No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA390.10963987517921903No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG380.10682859632846983No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT380.10682859632846983No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA380.10682859632846983No Hit
GGACTAACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTG370.10401731747772061No Hit
AACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTCGAAGA370.10401731747772061No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT360.1012060386269714No Hit
CTCATACACCTCCTATGAAAAAACTTCCTACCACTCACCCTAGCATTACT360.1012060386269714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)