FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008164918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008164918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13743
Total Bases2.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3472.524921778359892No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3442.503092483446118No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2001.4552863275849524No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1220.8877246598268209No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1130.822236775085498No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1010.734919595430401No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA930.6767081423270028No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA830.6039438259477553No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA810.5893909626719057No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT780.5675616677581314No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA760.5530088044822818No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC760.5530088044822818No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT730.5311795095685077No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC710.5166266462926581No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA690.5020737830168085No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT690.5020737830168085No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG600.43658589827548566No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT570.41475660336171144No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG570.41475660336171144No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT560.40748017172378664No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT560.40748017172378664No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG560.40748017172378664No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT490.35654515025831335No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT490.35654515025831335No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC470.34199228698246376No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC440.32016299206868953No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG430.31288656043076474No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC410.29833369715491526No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG410.29833369715491526No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC390.2837808338790657No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG390.2837808338790657No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG390.2837808338790657No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT390.2837808338790657No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG390.2837808338790657No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC390.2837808338790657No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC380.2765044022411409No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT370.2692279706032162No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT360.26195153896529144No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG360.26195153896529144No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT330.24012224405151714No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA330.24012224405151714No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG330.24012224405151714No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA320.23284581241359237No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG310.22556938077566763No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT310.22556938077566763No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC300.21829294913774283No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC300.21829294913774283No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG300.21829294913774283No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA300.21829294913774283No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA300.21829294913774283No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT290.21101651749981812No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT290.21101651749981812No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC290.21101651749981812No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA290.21101651749981812No Hit
AGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATATTGAATATACT280.20374008586189332No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC280.20374008586189332No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG280.20374008586189332No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC270.19646365422396855No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG270.19646365422396855No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT270.19646365422396855No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA270.19646365422396855No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT270.19646365422396855No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC260.18918722258604379No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG260.18918722258604379No Hit
CCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTG260.18918722258604379No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT250.18191079094811904No Hit
CTCGTAGACGGCATCTCACAGTCACTGAAGGCTATTAAAGAGTTAGCACC250.18191079094811904No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA240.17463435931019428No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT240.17463435931019428No Hit
AGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTA240.17463435931019428No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG230.16735792767226954No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC230.16735792767226954No Hit
ATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTT230.16735792767226954No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG230.16735792767226954No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT220.16008149603434477No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT210.15280506439642No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT210.15280506439642No Hit
CCATTGGATGAAGCCCAGGATAAGTGACCCCCCCGGACCTTGGAGTTTCA210.15280506439642No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG210.15280506439642No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA210.15280506439642No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT210.15280506439642No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA210.15280506439642No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC210.15280506439642No Hit
GTTTAGGGGCCTGCCGTCAGCTACAATTCATGCTTGAAGGTTTCACTAGA210.15280506439642No Hit
GTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGG210.15280506439642No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA200.14552863275849523No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA200.14552863275849523No Hit
TCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCT200.14552863275849523No Hit
GGTCAGAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATA200.14552863275849523No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT200.14552863275849523No Hit
GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT190.13825220112057046No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA190.13825220112057046No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC190.13825220112057046No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT190.13825220112057046No Hit
ATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAAT190.13825220112057046No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC190.13825220112057046No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT190.13825220112057046No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG190.13825220112057046No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC190.13825220112057046No Hit
CTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCCAATGTATGGTCA190.13825220112057046No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA190.13825220112057046No Hit
CTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGC180.13097576948264572No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA180.13097576948264572No Hit
ACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAAT180.13097576948264572No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC180.13097576948264572No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC180.13097576948264572No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG180.13097576948264572No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA180.13097576948264572No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG180.13097576948264572No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT170.12369933784472095No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT170.12369933784472095No Hit
GTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGATTT170.12369933784472095No Hit
CCTTATGGGTTACCCCTCGCGAGTGCCATCGCATCGACTGACACACCTGC170.12369933784472095No Hit
AATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGT170.12369933784472095No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA170.12369933784472095No Hit
ATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGGATCACATTATG170.12369933784472095No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA170.12369933784472095No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC170.12369933784472095No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT170.12369933784472095No Hit
ATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCC170.12369933784472095No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT170.12369933784472095No Hit
ATTAAAGAGTTAGCACCCACCATTGGATGAAGCCCAGGATAAGTGACCCC160.11642290620679618No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG160.11642290620679618No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC160.11642290620679618No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT160.11642290620679618No Hit
CTTCTGGGTCTGCTGACAAAGCATGATCAGGACCTTCCATATTTTTATCT150.10914647456887142No Hit
CTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGG150.10914647456887142No Hit
CGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTCGCGGTTACCG150.10914647456887142No Hit
TTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCC150.10914647456887142No Hit
GGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATT150.10914647456887142No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT150.10914647456887142No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG150.10914647456887142No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG150.10914647456887142No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC150.10914647456887142No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC150.10914647456887142No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC150.10914647456887142No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC150.10914647456887142No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT150.10914647456887142No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT150.10914647456887142No Hit
CAATAACATCCGCTCCTAAGGCAACAGAAACTACTGGGGCGAGTATTCCC140.10187004293094666No Hit
CCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGG140.10187004293094666No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC140.10187004293094666No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA140.10187004293094666No Hit
GGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATA140.10187004293094666No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG140.10187004293094666No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT140.10187004293094666No Hit
ATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT140.10187004293094666No Hit
TGTATGGTCAGAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTT140.10187004293094666No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC140.10187004293094666No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC140.10187004293094666No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT140.10187004293094666No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG140.10187004293094666No Hit
GTTATATGGAATCATTGGAGTGCCAGTAATTCTACCACGCCTTATATTTA140.10187004293094666No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)