FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008165185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008165185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651370
Total Bases284.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC150030.9085183817073097No Hit
GTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACA135900.8229530632141798No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA90750.5495437121904843No Hit
CCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTC89880.5442753592471705No Hit
CCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTC82260.4981318541574571No Hit
CTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTAT79390.4807523450226176No Hit
GTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCACCCAGCGCTG55470.3359029169719687No Hit
CCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTT55230.33444957822898563No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT53120.32167230844692585No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC51700.31307338755094255No Hit
TCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATTATGCTAATGTT50440.3054433591502813No Hit
CTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTT48210.29193941999672995No Hit
GCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTC46120.27928326177658547No Hit
GGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAA42320.25627206501268646No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41280.249974263793093No Hit
GGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCTCTCGCG40890.24761258833574548No Hit
CCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGAC40540.24549313600222847No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA35110.21261134694223585No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT34720.2102496714848883No Hit
ATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTC31050.18802569987343842No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC29190.1767623246153194No Hit
GCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCC28920.17512731852946342No Hit
CTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGA28570.17300786619594638No Hit
TTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCA28570.17300786619594638No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT28300.17137286011009042No Hit
GCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAG27780.1682239595002937No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCC27530.16671006497635296No Hit
GGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCT27360.16568061670007328No Hit
CCTCCAGCTCATCTTTCACCTCACCCCCCTCCTCCTCCTTGGCTTTAATT26680.1615628235949545No Hit
GCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTC26320.15938281548047983No Hit
CAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTT26280.15914059235664932No Hit
CAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAG25820.1563550264325984No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24440.14799832866044557No Hit
GACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTC23560.1426694199361742No Hit
GCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGAC22780.13794606902147916No Hit
GAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCC22140.1340704990401909No Hit
GGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAG21910.1326777160781654No Hit
CCGTGATGCATGAGGGTCTGCACAACCACTACACGCAGAAGAGCCTCTCC21270.12880214609687715No Hit
GTGCTGGACTCCGACGGCTCCTTCTTCCTCTACAGCAAGCTCACCGTGGA21190.12831769984921612No Hit
GTCACAAAGAGCTTCAACAGGGGAGAGTGTTAGAGGGAGAAGTGCCCCCA20390.12347323737260578No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20080.12159600816291927No Hit
CCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCG19400.1174782150578005No Hit
GGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAATAAAGTGAATCT19250.11656987834343605No Hit
ACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCCCTCC19240.11650932256247844No Hit
GACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTAC19190.11620654365769027No Hit
CACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGA19090.11560098584811399No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA18600.11263375258119017No Hit
CTCCTCCTCCTTGGCTTTAATTATGCTAATGTTGGAGGAGAATGAATAAA18440.1116648600858681No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT18420.11154374852395284No Hit
TGTTGGAGGAGAATGAATAAATAAAGTGAATCTTTGC18380.11130152540012232No Hit
CACGTACCCCGTGTACATACTTCCCGGGCGCCCAGCATGGAAATAAAGCA18220.11033263290480026No Hit
AGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCA18080.10948485197139345No Hit
GGGGAGAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGC18040.10924262884756293No Hit
GTCTCCGGGTAAATGAGTGCGACGGCCGGCAAGCCCCCGCTCCCCGGGCT17760.10754706698074931No Hit
GGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTTTCACCTCACCCCC17550.10627539558063911No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG17370.10518539152340178No Hit
GCAATGGGCAGCCGGAGAACAACTACAAGACCACGCCTCCCGTGCTGGAC17370.10518539152340178No Hit
CTGCACAACCACTACACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATG17220.10427705480903733No Hit
GTCTTCTCATGCTCCGTGATGCATGAGGGTCTGCACAACCACTACACGCA17210.10421649902807972No Hit
CCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGGACCTACCCCTATTG17170.10397427590424921No Hit
CCCTTTTTCCACAGGGGACCTACCCCTATTGCGGTCCTCCAGCTCATCTT17060.10330816231371527No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACT16860.10209704669456268No Hit
ACGCAGAAGAGCCTCTCCCTGTCTCCGGGTAAATGAGTGCGACGGCCGGC16840.10197593513264742No Hit
CACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGT16800.10173371200881691No Hit
GAGTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGAC16730.10130982154211352No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)