FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008165237

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008165237
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28007
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACATGGGGGGG2370.8462170171742778No Hit
GTATCAACGCAGAGTACATGGGGGG2320.8283643374870568No Hit
GTATCAACGCAGAGTACATGGGGGGG2290.8176527296747242No Hit
GGTATCAACGCAGAGTACATGGGGG1880.6712607562395115No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1820.6498375406148462No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1560.5570036062412969No Hit
GTATCAACGCAGAGTACATGGGGAG820.29278394687042525No Hit
GTATCAACGCAGAGTACATGGGGTG700.24993751562109473No Hit
GGTATCAACGCAGAGTACATGGGGGGG660.23565537187131788No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC620.22137322812154103No Hit
GGTATCAACGCAGAGTACATGGGGGG590.2106616203092084No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA580.2070910843717642No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC560.1999500124968758No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA550.19637947655943158No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT510.18209733280965473No Hit
GTATCAACGCAGAGTACATGGGATG500.1785267968722105No Hit
TATCAACGCAGAGTACATGGGGGGGACCTCC490.17495626093476632No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG480.1713857249973221No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA420.14996250937265682No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG410.14639197343521262No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA400.14282143749776843No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT400.14282143749776843No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT390.13925090156032421No Hit
GTATCAACGCAGAGTACATGGGAGG390.13925090156032421No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG380.13568036562288No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG370.13210982968543578No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT360.12853929374799158No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT340.12139822187310315No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG320.11425714999821472No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC320.11425714999821472No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA320.11425714999821472No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA310.11068661406077052No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC310.11068661406077052No Hit
GTATCAACGCAGAGTACATGGAGAG300.1071160781233263No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT300.1071160781233263No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC290.1035455421858821No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT290.1035455421858821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)