FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008165285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008165285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23108
Total Bases3.3 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2050.8871386532802493No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1900.8222260688938896No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA890.3851480006924009No Hit
GGTATCAACGCAGAGTACATGGGGG850.36783797818937164No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT820.35485546131209966No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG810.3505279556863424No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC800.3462004500605851No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG730.31590791068028384No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA720.31158040505452655No Hit
GTATCAACGCAGAGTACATGGGGGG710.30725289942876927No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT690.29859788817725463No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG680.29427038255149734No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT620.26830534879695345No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA600.2596503375454388No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG520.22503029253938028No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC460.1990652587848364No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT450.1947377531590791No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG420.18175523628180718No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT410.17742773065604983No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT410.17742773065604983No Hit
GTATCAACGCAGAGTACATGGGGAG390.16877271940453523No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG390.16877271940453523No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC390.16877271940453523No Hit
GTATCAACGCAGAGTACATGGGATTG370.1601177081530206No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG370.1601177081530206No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA370.1601177081530206No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG350.15146269690150596No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG330.14280768564999136No Hit
TATCAACGCAGAGTACATGGGATTG330.14280768564999136No Hit
GTATCAACGCAGAGTACATGGGGTG320.138480180024234No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA320.138480180024234No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC320.138480180024234No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT290.1254976631469621No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT290.1254976631469621No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA280.12117015752120479No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT280.12117015752120479No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT270.11684265189544746No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG270.11684265189544746No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC260.11251514626969014No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA260.11251514626969014No Hit
GCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTA260.11251514626969014No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA250.10818764064393284No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT250.10818764064393284No Hit
GTATCAACGCAGAGTACATGGGAGG250.10818764064393284No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG250.10818764064393284No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC240.10386013501817552No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG240.10386013501817552No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT240.10386013501817552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)