FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008165330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008165330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23317
Total Bases3.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4151.779817300681906No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2981.278037483381224No Hit
GTATCAACGCAGAGTACATGGGGGG1650.707638203885577No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1600.6861946219496505No Hit
GGTATCAACGCAGAGTACATGGGGG1470.6304413089162414No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1180.5060685336878672No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1000.42887163871853157No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA960.4117167731697903No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC920.39456190762104904No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG850.3645408929107518No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA800.34309731097482526No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA730.31307629626452804No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT720.30878757987734273No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT680.29163271432860144No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT640.2744778487798602No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC620.2659004160054895No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA570.244456834069563No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA520.2230132521336364No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG520.2230132521336364No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT510.21872453574645107No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG490.21014710297208045No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG490.21014710297208045No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC490.21014710297208045No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT480.20585838658489514No Hit
GTATCAACGCAGAGTACATGGGGAG470.20156967019770983No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT470.20156967019770983No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT470.20156967019770983No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA460.19728095381052452No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA450.1929922374233392No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG440.18870352103615387No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG420.18012608826178325No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT420.18012608826178325No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT400.17154865548741263No Hit
GGTATCAACGCAGAGTACATGGGGGG400.17154865548741263No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG380.16297122271304199No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC380.16297122271304199No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG370.15868250632585665No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT360.15439378993867137No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC360.15439378993867137No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT350.15010507355148606No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG350.15010507355148606No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT330.1415276407771154No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA320.1372389243899301No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT320.1372389243899301No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG310.13295020800274476No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC300.12866149161555948No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG300.12866149161555948No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC300.12866149161555948No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG290.12437277522837414No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG290.12437277522837414No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT280.12008405884118883No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA280.12008405884118883No Hit
GCGTTAGACCCCGTGACTCGTGGCTGAACGGCCGTACAACTCGACAGCCG280.12008405884118883No Hit
CCTTAATATATTCGCTGGTTTAACTGACGAAGCTCAACATGCTGAAATCA280.12008405884118883No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG280.12008405884118883No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG280.12008405884118883No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC270.11579534245400352No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC270.11579534245400352No Hit
GTATCAACGCAGAGTACATGGGGTG260.1115066260668182No Hit
GTATCAACGCAGAGTACATGGAGAG260.1115066260668182No Hit
TGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTG260.1115066260668182No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT250.10721790967963289No Hit
GTATCAACGCAGAGTACATGGGAGG250.10721790967963289No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT250.10721790967963289No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG250.10721790967963289No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC250.10721790967963289No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT240.10292919329244757No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT240.10292919329244757No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC240.10292919329244757No Hit
GTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAG240.10292919329244757No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)