FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008165574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008165574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25719
Total Bases4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC3601.3997433803802635No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC3391.318091683191415No Hit
GGTATCAACGCAGAGTACATGGGGG2661.0342548310587503No Hit
GTATCAACGCAGAGTACATGGGGGG1820.7076480423033554No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1490.5793382324351647No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1330.5171274155293751No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1160.4510284225669738No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA1010.3927057817177962No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT900.34993584509506587No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG890.346047669038454No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA880.34215949298184223No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT860.33438314086861853No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT840.32660678875539484No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG820.31883043664217114No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT810.3149422605855593No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA810.3149422605855593No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT740.28772502818927637No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA720.2799486760760527No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT720.2799486760760527No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC680.26439597184960534No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG660.25661961973638164No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG650.25273144367976985No Hit
GGTATCAACGCAGAGTACATGGGGGG640.248843267623158No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG640.248843267623158No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT620.24106691550993428No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA590.22940238734009874No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA550.21384968311365138No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA550.21384968311365138No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT540.20996150705703953No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT530.20607333100042768No Hit
GTATCAACGCAGAGTACATGGGGAG520.20218515494381584No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA520.20218515494381584No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT520.20218515494381584No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA520.20218515494381584No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC520.20218515494381584No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG510.198296978887204No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT480.1866324507173685No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG480.1866324507173685No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT480.1866324507173685No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT480.1866324507173685No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT480.1866324507173685No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT470.18274427466075666No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT470.18274427466075666No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA460.1788560986041448No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC450.17496792254753293No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA440.17107974649092111No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG430.16719157043430927No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA430.16719157043430927No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG430.16719157043430927No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC420.16330339437769742No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC410.15941521832108557No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT410.15941521832108557No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT410.15941521832108557No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG390.1516388662078619No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG390.1516388662078619No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC390.1516388662078619No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC390.1516388662078619No Hit
GGCGCATAGTCGCCATTCACAGATTTGCTCGGCAATCAGTACTGGTAGGC370.14386251409463818No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG370.14386251409463818No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC370.14386251409463818No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC360.13997433803802636No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA360.13997433803802636No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT350.13608616198141452No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC350.13608616198141452No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC350.13608616198141452No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC340.13219798592480267No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG340.13219798592480267No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA340.13219798592480267No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC330.12830980986819082No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA330.12830980986819082No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA320.124421633811579No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG320.124421633811579No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC320.124421633811579No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC310.12053345775496714No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA310.12053345775496714No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG310.12053345775496714No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT300.1166452816983553No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC300.1166452816983553No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC300.1166452816983553No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG290.11275710564174345No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA290.11275710564174345No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG290.11275710564174345No Hit
GTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCT290.11275710564174345No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC290.11275710564174345No Hit
GCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCG290.11275710564174345No Hit
GTATCAACGCAGAGTACATGGGGAAG280.10886892958513163No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC280.10886892958513163No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG280.10886892958513163No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC280.10886892958513163No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG280.10886892958513163No Hit
TTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGA280.10886892958513163No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC270.10498075352851977No Hit
CTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCT270.10498075352851977No Hit
TATCAACGCAGAGTACATGGGGAAG270.10498075352851977No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA260.10109257747190792No Hit
TATCAACGCAGAGTACATGGGGGGG260.10109257747190792No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC260.10109257747190792No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA260.10109257747190792No Hit
GTATCAACGCAGAGTACATGGGAGG260.10109257747190792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)