FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008165782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008165782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24929
Total Bases4.4 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC4841.9415138994745076No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC4351.7449556741144852No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2170.8704721408800995No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2090.8383810020458101No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1580.6337999919772153No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT1410.5656063219543503No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1400.5615949296000642No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1390.557583537245778No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1210.4853784748686269No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1100.441253158971479No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1070.4292189819086205No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG1040.417184804845762No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1040.417184804845762No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC1030.4131734124914758No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC930.37305948894861407No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA920.36904809659432786No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG810.32492278069718No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT770.3088772112800353No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT760.30486581892574915No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG760.30486581892574915No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT750.300854426571463No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG750.300854426571463No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA690.27678607244574593No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT650.2607405030286012No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT640.25672911067431503No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG640.25672911067431503No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG630.2527177183200289No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT610.24469493361145653No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC600.24068354125717037No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG570.22864936419431187No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA570.22864936419431187No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC550.2206265794857395No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG550.2206265794857395No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG540.21661518713145334No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT520.208592402422881No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG510.20458101006859483No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG500.20056961771430862No Hit
GGTCATGCTGTTGGTGATATTCCTGGAGTCCGCTTTAAGGTTGTCAAAGT490.19655822536002246No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC490.19655822536002246No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG480.1925468330057363No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT470.18853544065145011No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC470.18853544065145011No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA470.18853544065145011No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA470.18853544065145011No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG460.18452404829716393No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT450.18051265594287777No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT440.1765012635885916No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT440.1765012635885916No Hit
GCAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGT430.17248987123430543No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC430.17248987123430543No Hit
GGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGAT420.16847847888001927No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC420.16847847888001927No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT420.16847847888001927No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC410.16446708652573308No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC400.1604556941714469No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC400.1604556941714469No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG400.1604556941714469No Hit
GATTTAATTCTGCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACA400.1604556941714469No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA390.15644430181716074No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA380.15243290946287458No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA370.1484215171085884No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG350.14039873240001605No Hit
AATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGA350.14039873240001605No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT340.13638734004572986No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT340.13638734004572986No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA340.13638734004572986No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC340.13638734004572986No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA330.1323759476914437No Hit
GCATACCCCCGATTCCGCTACGACCAACTCATACACCTCCTATGAAAAAA330.1323759476914437No Hit
GCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCC330.1323759476914437No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG330.1323759476914437No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT320.12836455533715752No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA320.12836455533715752No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC320.12836455533715752No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA310.12435316298287134No Hit
TCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACA310.12435316298287134No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA300.12034177062858518No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG300.12034177062858518No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG300.12034177062858518No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT300.12034177062858518No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC300.12034177062858518No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC300.12034177062858518No Hit
ATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGC300.12034177062858518No Hit
CAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGA290.11633037827429901No Hit
CCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTT290.11633037827429901No Hit
GTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGGCCGC290.11633037827429901No Hit
CAATAACACAGGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTT290.11633037827429901No Hit
GGGCACTGGCCCCCAACAGGCATCACCCCGCTAAATCCCCTAGAAGTCCC280.11231898592001283No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC280.11231898592001283No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA280.11231898592001283No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT280.11231898592001283No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC280.11231898592001283No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT280.11231898592001283No Hit
GTTAAGAGCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATC270.10830759356572667No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA270.10830759356572667No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC270.10830759356572667No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC270.10830759356572667No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT270.10830759356572667No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC270.10830759356572667No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA270.10830759356572667No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG270.10830759356572667No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG260.1042962012114405No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC260.1042962012114405No Hit
GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT260.1042962012114405No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT260.1042962012114405No Hit
GGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGCAACATCATATACCC260.1042962012114405No Hit
GCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAAC260.1042962012114405No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG260.1042962012114405No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG250.10028480885715431No Hit
GTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGAC250.10028480885715431No Hit
CTCCCTGCCACATTGAGATGATCCTTACGGAAAAGGAACAGATTGTTCCT250.10028480885715431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)