FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008166021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008166021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577773
Total Bases277.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG39840.25250780688983776No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC24730.15673991125466086No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA23850.15116242957637127No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA20980.13297223364831318No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC20550.13024687328278528No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA18250.11566936435089205No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT16550.10489468383601444No Hit
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC16240.10292988915388969No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)