FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008166369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008166369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9348
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2342.503209242618742No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1701.8185708172871202No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC951.0162601626016259No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA750.8023106546854942No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT600.6418485237483954No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA590.6311510483525888No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA590.6311510483525888No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA560.599058622165169No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT530.5669661959777492No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT530.5669661959777492No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG500.5348737697903295No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC490.5241762943945228No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT470.5027813436029097No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG460.4920838682071031No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT450.4813863928112966No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT420.4492939666238768No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG390.41720154043645696No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG390.41720154043645696No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG350.37441163885323064No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA340.363714163457424No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG340.363714163457424No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC320.3423192126658109No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT320.3423192126658109No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG310.33162173727000427No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA300.3209242618741977No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC300.3209242618741977No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG300.3209242618741977No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC260.2781343602909713No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG260.2781343602909713No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT250.26743688489516476No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT250.26743688489516476No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT240.25673940949935814No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC240.25673940949935814No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT240.25673940949935814No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC230.24604193410355155No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT230.24604193410355155No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT230.24604193410355155No Hit
GCTTGAAGCAGATGATATCCCGATGCTGACGGCTTACAATAAACGTGATC220.23534445870774498No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG220.23534445870774498No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT210.2246469833119384No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG210.2246469833119384No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC210.2246469833119384No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC210.2246469833119384No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC210.2246469833119384No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC200.21394950791613182No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA200.21394950791613182No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT190.20325203252032523No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT190.20325203252032523No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC190.20325203252032523No Hit
ATACCAAAGAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTC190.20325203252032523No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG190.20325203252032523No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC180.19255455712451863No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA180.19255455712451863No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC180.19255455712451863No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG180.19255455712451863No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC180.19255455712451863No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT180.19255455712451863No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA160.17115960633290545No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG160.17115960633290545No Hit
GCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCG160.17115960633290545No Hit
TTCCTATTGTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAAT160.17115960633290545No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC160.17115960633290545No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT160.17115960633290545No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT160.17115960633290545No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT150.16046213093709885No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA150.16046213093709885No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT150.16046213093709885No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA150.16046213093709885No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG150.16046213093709885No Hit
GCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTC150.16046213093709885No Hit
ATCATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAG150.16046213093709885No Hit
ATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGG150.16046213093709885No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT140.14976465554129226No Hit
ATATGAGATAATATCCTTATCTTGAGACGCATAGCAAATGTAGGAGAGAG140.14976465554129226No Hit
GTCCATCAGTTGTCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTT140.14976465554129226No Hit
CTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTC140.14976465554129226No Hit
GTACATGGGCGTCTCAGGTCGGTCTCACAGC140.14976465554129226No Hit
ATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGG140.14976465554129226No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG140.14976465554129226No Hit
GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG140.14976465554129226No Hit
GCTCCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAG140.14976465554129226No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC140.14976465554129226No Hit
GCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCT140.14976465554129226No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT130.13906718014548566No Hit
TCCGTATACCAAGACGTCTAAGGGCGGTGTACACCCTTTTGAGCAATGAT130.13906718014548566No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC130.13906718014548566No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA130.13906718014548566No Hit
GCATTTAATTGATTCTTCAAATGAGGATTATGCGGGACATGAAAAAACAG130.13906718014548566No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC130.13906718014548566No Hit
CCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCC130.13906718014548566No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC130.13906718014548566No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC130.13906718014548566No Hit
CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC130.13906718014548566No Hit
TCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACT130.13906718014548566No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT120.12836970474967907No Hit
GTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAA120.12836970474967907No Hit
ACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATC120.12836970474967907No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG120.12836970474967907No Hit
GAATATCCCACAGGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTT120.12836970474967907No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA120.12836970474967907No Hit
GTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCCCGATGCTGAC120.12836970474967907No Hit
GCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAG120.12836970474967907No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG120.12836970474967907No Hit
CTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTT120.12836970474967907No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC120.12836970474967907No Hit
GTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATTTCTGATATTTCC120.12836970474967907No Hit
AGCATGATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAA120.12836970474967907No Hit
GGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGCTTATGA110.11767222935387249No Hit
ATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAA110.11767222935387249No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA110.11767222935387249No Hit
CCCCGAGGAGCGGACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGG110.11767222935387249No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT110.11767222935387249No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA110.11767222935387249No Hit
ATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTAT110.11767222935387249No Hit
TCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTGATTTT110.11767222935387249No Hit
TGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCCTG110.11767222935387249No Hit
GGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTG110.11767222935387249No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC110.11767222935387249No Hit
CATATACCCTGTAGAACCCGACTTTTGGGTTTAGGGGCCTGCCGTCAGCT100.10697475395806591No Hit
GATCATGTTTATTGGAAGCATCAACCTGCGCCGTCTTGTTAACTTGTCAT100.10697475395806591No Hit
GGGGCATACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGG100.10697475395806591No Hit
CACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACT100.10697475395806591No Hit
ATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAAAACTTT100.10697475395806591No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG100.10697475395806591No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT100.10697475395806591No Hit
CATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCAGCATA100.10697475395806591No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA100.10697475395806591No Hit
ACGCTTGAGGAAGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTC100.10697475395806591No Hit
AGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCA100.10697475395806591No Hit
GTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAACAGAAACTACTGG100.10697475395806591No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC100.10697475395806591No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA100.10697475395806591No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC100.10697475395806591No Hit
GCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTACTCATA100.10697475395806591No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA100.10697475395806591No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT100.10697475395806591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)