FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008166548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008166548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3409257
Total Bases597.9 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACC113920.33414905359144237No Hit
TTGCCACACTGAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAG93840.2752505897912654No Hit
ACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGCCTTTAGTGAT87890.25779810674290615No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG59130.17343955002512276No Hit
GGCTAATGCCCTGGCCCACAAGTATCACTAAGCTCGCTTTCTTGCTGTCC55350.16235209020616514No Hit
GTTATGGGCAACCCTAAGGTGAAGGCTCATGGCAAGAAAGTGCTCGGTGC54470.15977088262926498No Hit
GTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCAACGTG54460.159741550724982No Hit
CTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCC47720.13997184723826922No Hit
GCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGCCCACAAGTA47420.1390918901097805No Hit
GAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCT46510.13642268682003147No Hit
GTCCACTCCTGATGCTGTTATGGGCAACCCTAAGGTGAAGGCTCATGGCA46310.13583604873437233No Hit
CTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGA44640.13093762071911855No Hit
GTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGCAGGCTGC41930.1229886746584373No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA41210.12087677755006443No Hit
GGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTCACCCCACCAGTGC41070.12046613089010302No Hit
CACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCC40220.11797291902605173No Hit
GTGACAAGCTGCATGTGGATCCTGAGAACTTCAAGCTCCTGGGAAATGTG39660.11633033238620613No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC38260.11222386578659221No Hit
GAATTCACCCCACCAGTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGT37700.11058127914674663No Hit
GTGCAGGCTGCCTATCAGAAAGTGGTGGCTGGTGTGGCTAATGCCCTGGC36640.10747209729275323No Hit
GCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGCTCCTGGGCA35000.10266166499034834No Hit
GAGTGAGCTGCACTGTGACAAGCTGCACGTGGATCCTGAGAACTTCAGGC34760.10195769928755738No Hit
GTGCCTTTAGTGATGGCCTGGCTCACCTGGACAACCTCAAGGGCACCTTT34440.10101907835050275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)