FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008166753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008166753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12179
Total Bases2.1 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC1801.4779538549963052No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC1701.3958453074965105No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT960.7882420559980294No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC960.7882420559980294No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT810.6650792347483373No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA710.5829706872485425No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA670.5501272682486247No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT590.4844404302487889No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG510.4187535922489531No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG510.4187535922489531No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC510.4187535922489531No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT500.41054273749897363No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA480.3941210279990147No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT470.38591017324903526No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA460.37769931849905575No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA440.36127760899909683No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG430.3530667542491173No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG420.34485589949913786No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA380.31201248049922No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG360.295590770999261No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT330.27095820674932264No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG330.27095820674932264No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG320.2627473519993431No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT320.2627473519993431No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT320.2627473519993431No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG320.2627473519993431No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC310.25453649724936367No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA300.24632564249938418No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC300.24632564249938418No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT300.24632564249938418No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC300.24632564249938418No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG290.23811478774940473No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG280.22990393299942524No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG270.22169307824944579No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC270.22169307824944579No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG270.22169307824944579No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG260.21348222349946627No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA250.20527136874948682No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT250.20527136874948682No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT230.18884965924952787No Hit
GATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACA230.18884965924952787No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT230.18884965924952787No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT220.18063880449954842No Hit
GGGATATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATC220.18063880449954842No Hit
GGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTG220.18063880449954842No Hit
TGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAGTC210.17242794974956893No Hit
ATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGACCGC210.17242794974956893No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG210.17242794974956893No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA200.16421709499958945No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC200.16421709499958945No Hit
GAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTGACATCTTCATAAG190.15600624024961No Hit
GTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCG190.15600624024961No Hit
GTTATGGGCTATTACATCTCTAAGGAATTGGTGAAGTAAAATGGTGAAGC190.15600624024961No Hit
GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT190.15600624024961No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT180.1477953854996305No Hit
GCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTT180.1477953854996305No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC180.1477953854996305No Hit
GAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTTACTGCGTCACGCT180.1477953854996305No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC180.1477953854996305No Hit
CAGTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGAT170.13958453074965105No Hit
GCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCAC170.13958453074965105No Hit
CATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATC170.13958453074965105No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT160.13137367599967156No Hit
ATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAAC160.13137367599967156No Hit
GTTTTAATAGCGTTTAAATTGACATCTTCATAAGGGGTTGGGTAAGATGA160.13137367599967156No Hit
GCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCT160.13137367599967156No Hit
CTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAGTAATT160.13137367599967156No Hit
GTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGG160.13137367599967156No Hit
GGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATT160.13137367599967156No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC160.13137367599967156No Hit
CATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCA160.13137367599967156No Hit
GCCTAAAGGTTCTTGCAGAGCAACATCATATACCCTGTAGAACCCGACTT150.12316282124969209No Hit
GGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCCCTGCATCCA150.12316282124969209No Hit
TAATATCCCTGCATCCATTATTTTCTAAAACTTTAACTGCTTCCCAAATT150.12316282124969209No Hit
TTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTG150.12316282124969209No Hit
ATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAGTTGT150.12316282124969209No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA150.12316282124969209No Hit
ATATCAGACATTCCTGTAGATAAAATCACCGGCTTGCCTGTTTTTGCCAC150.12316282124969209No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT150.12316282124969209No Hit
GTCTCGACGGGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATT150.12316282124969209No Hit
CATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTTGCTCGGCAAT140.11495196649971262No Hit
ATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAA140.11495196649971262No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT140.11495196649971262No Hit
TGATTGCACAACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCC140.11495196649971262No Hit
CTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGGCG140.11495196649971262No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC140.11495196649971262No Hit
CTTCAAATGAGGATTATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTT140.11495196649971262No Hit
ACCTGCGATCACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGG140.11495196649971262No Hit
GCCTTATATTTAAAGACAAAAAGAAGTGTCTAACAACAACATCCACACTA140.11495196649971262No Hit
CTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACC140.11495196649971262No Hit
GTTGTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGC140.11495196649971262No Hit
GCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGT140.11495196649971262No Hit
GCCCTATACTCCCTCTACATATTTACCACAACACAATGGGGCTCACTCAC140.11495196649971262No Hit
CTACTGGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATA140.11495196649971262No Hit
CCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATA140.11495196649971262No Hit
GTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGAGCGCCAGGGG140.11495196649971262No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT130.10674111174973314No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA130.10674111174973314No Hit
CATCCACACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTT130.10674111174973314No Hit
GGTCATTGAGCATATCCAAGTGAACAAAGCACCTAAGATGCGCCGCCGGA130.10674111174973314No Hit
CACTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGA130.10674111174973314No Hit
CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA130.10674111174973314No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG130.10674111174973314No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)