FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34869
Total Bases5.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC2700.7743267658952078No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC2430.696894089305687No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA1090.3125985832688061No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC1070.3068628294473601No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA830.23803378359000832No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA800.22943015285783935No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT780.22369439903639335No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG760.21795864521494734No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA760.21795864521494734No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC750.21509076830422438No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA740.2122228913935014No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA720.2064871375720554No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT720.2064871375720554No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA720.2064871375720554No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC690.19788350683988642No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT690.19788350683988642No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG680.19501562992916344No Hit
CTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTCAG670.19214775301844045No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC640.18354412228627148No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG640.18354412228627148No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA640.18354412228627148No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA630.18067624537554847No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA630.18067624537554847No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT620.1778083684648255No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA620.1778083684648255No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT610.1749404915541025No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG600.1720726146433795No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC580.16633686082193352No Hit
GTCTAAGGGCGGTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGAT570.16346898391121054No Hit
GACTTGTAAGGACCGCCGCTTTCGCTCGGGTCTGCGGGTTATAGCTTTTC560.16060110700048755No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG560.16060110700048755No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA550.15773323008976453No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG530.15199747626831855No Hit
GATGAAATACAATGCAATAAAATAATATCCCTGCATCCATTATTTTCTAA530.15199747626831855No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA510.14626172244687258No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC490.1405259686254266No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA480.1376580917147036No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT470.13479021480398062No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT460.13192233789325764No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG460.13192233789325764No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT450.12905446098253462No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT450.12905446098253462No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG450.12905446098253462No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT440.12618658407181166No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA430.12331870716108864No Hit
ATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCAT430.12331870716108864No Hit
CTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACAGAATTATCA430.12331870716108864No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG420.12045083025036567No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA410.11758295333964267No Hit
GTATTCTATAGCTCCATTATGGCCGTTATATGGAATCATTGGAGTGCCAG410.11758295333964267No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA400.11471507642891968No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT390.11184719951819667No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA380.10897932260747367No Hit
CATTATGGTCAGTGCGAAATTTGAGGACGACGCTGCAGCCTTTAAAGAAG380.10897932260747367No Hit
GTATCAACGCAGAGTACATGGGGGG360.1032435687860277No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA360.1032435687860277No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG350.1003756918753047No Hit
GATGATATCCCGATGCTGACGGCTTACAATAAACGTGATCAAAAACTGCC350.1003756918753047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)