FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56307
Total Bases7.5 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6481.1508338217273162No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5460.969684053492461No Hit
GGTATCAACGCAGAGTACATGGGGG4190.7441348322588666No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA4040.7174951604596231No Hit
GTATCAACGCAGAGTACATGGGGGG4030.7157191823396736No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC3640.6464560356616407No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC3450.612712451382599No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA3300.5860727795833555No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2970.5274655016250199No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA2430.4315626831477436No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA2250.3995950769886515No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG2170.385387252029055No Hit
CCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTA2100.37295540518940806No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2070.3676274708295594No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC2020.35874758022981157No Hit
GTACATGGGGAAAAAAAGAAAAGAAAAGAAAAG1790.31790008347097165No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1670.2965883460315769No Hit
GGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGA1670.2965883460315769No Hit
GCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTA1550.27527660859218217No Hit
CTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG1510.2681726961123839No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1470.2610687836325857No Hit
CATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAA1430.2539648711527874No Hit
GGTATCAACGCAGAGTACATGGGGGG1380.2450849805530396No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA1370.24330900243309003No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAGAAAAGAAAAGAAAAG1370.24330900243309003No Hit
CTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATT1350.23975704619319088No Hit
GTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCTAC1350.23975704619319088No Hit
GATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTG1290.22910117747349354No Hit
CCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATT1250.2219972649936953No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAGAAAAGAAAAGAAAAG1220.21666933063384658No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA1200.21311737439394748No Hit
CTACTACTCCTTGTCATATTTCTTCCTGAATTGCTGATTCTTATTGGATT1170.20778944003409877No Hit
GACAATTAACAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCC1140.2024615056742501No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT1110.19713357131440143No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA1100.19535759319445184No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA1100.19535759319445184No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG1090.19358161507450228No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT1080.19180563695455272No Hit
GCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCCTCA1050.18647770259470403No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT1050.18647770259470403No Hit
CACCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATA990.17582183387500666No Hit
ACCTTGTAGAGAGAGTAAAAAATTCAACACCCATAGTAGGCCTAAAAGCA930.1651659651553093No Hit
ACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAA920.16338998703535973No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA910.16161400891541017No Hit
GATTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCA880.15628607455556148No Hit
ACGCAGAGTACATGGGGAAAAAAAGAAAAGAAAAGAAAAG850.15095814019571277No Hit
CACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGC850.15095814019571277No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA840.14918216207576324No Hit
GTATCAACGCAGAGTACATGGGGAG830.14740618395581365No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG810.14385422771591452No Hit
CCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAA800.142078249595965No Hit
TAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACAC790.14030227147601543No Hit
CCTAATAGGAGGTGAATTAGATAGGGAAAAGATCGGGATGCTACTAGTTT790.14030227147601543No Hit
CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT780.13852629335606587No Hit
GTATCAACGCAGAGTACATGGGAGG750.13319835899621718No Hit
ATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAAAAATGGG750.13319835899621718No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC750.13319835899621718No Hit
CAATAAACGTGATCAAAAACTGCCTGATTTTATACCGACCGCCGGAAGGG740.1314223808762676No Hit
GGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTT710.12609444651641893No Hit
ACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTAC700.12431846839646934No Hit
CTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCT690.1225424902765198No Hit
GCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTCAAC690.1225424902765198No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA680.12076651215657024No Hit
GGCTAGCACACATCTGGTTGACTAGGCGCATAGTCGCCATTCACAGATTT670.11899053403662067No Hit
ATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACAC670.11899053403662067No Hit
ACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAG670.11899053403662067No Hit
ATTTTATACCGACCGCCGGAAGGGATCACATTATGGTCAGTGCGAAATTT660.11721455591667111No Hit
CCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTT660.11721455591667111No Hit
CATTATAACTAAAAATGGGATATATTCCTTATAAACTACTGCTAAGACAG660.11721455591667111No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT660.11721455591667111No Hit
CATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAAACTACT650.11543857779672154No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC650.11543857779672154No Hit
GGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCATCTTCTTTGAG640.11366259967677199No Hit
CTTCATAAGGGGTTGGGTAAGATGAAATACAATGCAATAAAATAATATCC640.11366259967677199No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT640.11366259967677199No Hit
ACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTC630.11188662155682241No Hit
CATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAG620.11011064343687287No Hit
GTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGAGAG620.11011064343687287No Hit
GGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGTTGACTAGG610.10833466531692329No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC600.10655868719697374No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC600.10655868719697374No Hit
GGCATCTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCT600.10655868719697374No Hit
GCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAAC600.10655868719697374No Hit
GCATCAACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGC590.10478270907702417No Hit
CTTGTAGAGAGAGTAAAAAATTCAACACCCATAGTAGGCCTAAAAGCAGC590.10478270907702417No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTT580.10300673095707462No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)