FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008167274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008167274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181289
Total Bases28.2 Mbp
Sequences flagged as poor quality0
Sequence length25-200
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC6010.331514874040896No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC5560.306692628896403No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC2670.14727865452399208No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG2530.13955617825681646No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA2410.13293691288495166No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA2370.13073049109433005No Hit
GTATTACAGAGCTCATTTGGTATTCCATTCTCTTTGGGTGGTACCTCTTT2340.12907567475136386No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA2110.11638874945528963No Hit
GTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA2070.11418232766466802No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC2010.11087269497873561No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC1950.1075630622928032No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA1820.10039219147328299No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1820.10039219147328299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)